长治医学院学报
長治醫學院學報
장치의학원학보
JOURNAL OF CHANGZHI MEDICAL COLLEGE
2011年
4期
241-244
,共4页
rHMGB1 A-box蛋白%HMGB1%LPS%TNF-α
rHMGB1 A-box蛋白%HMGB1%LPS%TNF-α
rHMGB1 A-box단백%HMGB1%LPS%TNF-α
目的:观察重组HMGB1 A-box蛋白对HMGB1的特异性拮抗作用.方法:复苏的RAW264.7细胞培养至对数生长期,用于以下实验:实验分为六组,rHMGB1组:加500 ng/mLrHMGB1；LPS组:加100 ng/mL LPS;A-box干预1组:加500 ng/mL rHMGB1和100 ng/mL A-box;A-box干预2组:加500 ng/mL rHMGB1和200 ng/mL A-box；A-box干预3组:加500 ng/mLrHMGB1和500 ng/mL A-box；A-box干预4组；加100 ng/mL LPS和500 ng/mL A-box.培养6h后,收集上清液,待测.各组上清液采用ELISA盒检测TNF-α的含量.结果:200 ng/mL、500 ng/mL重组A-box蛋白能明显抑制HMGB1诱导培养细胞释放TNF-α的水平(P＜0.05),但对LPS的无明显作用.结论:rHMGB1 A-box蛋白可能有特异性拮抗HMGB1的致炎作用.
目的:觀察重組HMGB1 A-box蛋白對HMGB1的特異性拮抗作用.方法:複囌的RAW264.7細胞培養至對數生長期,用于以下實驗:實驗分為六組,rHMGB1組:加500 ng/mLrHMGB1；LPS組:加100 ng/mL LPS;A-box榦預1組:加500 ng/mL rHMGB1和100 ng/mL A-box;A-box榦預2組:加500 ng/mL rHMGB1和200 ng/mL A-box；A-box榦預3組:加500 ng/mLrHMGB1和500 ng/mL A-box；A-box榦預4組；加100 ng/mL LPS和500 ng/mL A-box.培養6h後,收集上清液,待測.各組上清液採用ELISA盒檢測TNF-α的含量.結果:200 ng/mL、500 ng/mL重組A-box蛋白能明顯抑製HMGB1誘導培養細胞釋放TNF-α的水平(P＜0.05),但對LPS的無明顯作用.結論:rHMGB1 A-box蛋白可能有特異性拮抗HMGB1的緻炎作用.
목적:관찰중조HMGB1 A-box단백대HMGB1적특이성길항작용.방법:복소적RAW264.7세포배양지대수생장기,용우이하실험:실험분위륙조,rHMGB1조:가500 ng/mLrHMGB1；LPS조:가100 ng/mL LPS;A-box간예1조:가500 ng/mL rHMGB1화100 ng/mL A-box;A-box간예2조:가500 ng/mL rHMGB1화200 ng/mL A-box；A-box간예3조:가500 ng/mLrHMGB1화500 ng/mL A-box；A-box간예4조；가100 ng/mL LPS화500 ng/mL A-box.배양6h후,수집상청액,대측.각조상청액채용ELISA합검측TNF-α적함량.결과:200 ng/mL、500 ng/mL중조A-box단백능명현억제HMGB1유도배양세포석방TNF-α적수평(P＜0.05),단대LPS적무명현작용.결론:rHMGB1 A-box단백가능유특이성길항HMGB1적치염작용.
