CAJ | 학술논문

目的分析急性期和恢复期实验性结肠炎小鼠外周血Th1/Th2亚群比例以及青春双歧杆菌和嗜酸乳酸杆菌对其影响.方法 40只BABL/c小鼠自由饮用7d DSS造成急性实验性结肠炎小鼠模型,随机分为4组:阴性对照组(NS)、阳性对照组(SASP)、青春双歧杆菌组(BF0624)、嗜酸乳杆菌组(LT0637),10只/组,以及正常对照组(N)10只(未造模).一部分在实验第8天处死采血;另一部分继续正常饮水10 d,在实验第18天处死采血;分离淋巴细胞,用流式细胞术三色法测定Th1/Th2亚群比例.结果正常小鼠外周血Th1/Th2比例在0.84-0.96之间,而DSS诱导的急性结肠炎小鼠Th1/Th2比例增加,NS组达到2.21±0.83.在恢复期同样处于较高的水平;青春双歧杆菌和嗜酸乳杆菌能逐渐降低实验性UC小鼠的Th1/Th2比例,特别是嗜酸乳杆菌在恢复期可以使比例达到接近正常小鼠水平.结论 DSS诱导的急性期和恢复期结肠炎小鼠外周血Th1/Th2比例较正常小鼠增加,青春双歧杆菌和嗜酸乳杆菌能降低Th1/Th2比例,嗜酸乳酸杆菌效果更优.
목적 분석급성기화회복기실험성결장염소서외주혈Th1/Th2아군비례이급청춘쌍기간균화기산유산간균대기영향.방법 40지BABL/c소서자유음용7d DSS조성급성실험성결장염소서모형,수궤분위4조:음성대조조(NS)、양성대조조(SASP)、청춘쌍기간균조(BF0624)、기산유간균조(LT0637),10지/조,이급정상대조조(N)10지(미조모).일부분재실험제8천처사채혈;령일부분계속정상음수10 d,재실험제18천처사채혈;분리림파세포,용류식세포술삼색법측정Th1/Th2아군비례.결과 정상소서외주혈Th1/Th2비례재0.84-0.96지간,이DSS유도적급성결장염소서Th1/Th2비례증가,NS조체도2.21±0.83.재회복기동양처우교고적수평;청춘쌍기간균화기산유간균능축점강저실험성UC소서적Th1/Th2비례,특별시기산유간균재회복기가이사비례체도접근정상소서수평.결론 DSS유도적급성기화회복기결장염소서외주혈Th1/Th2비례교정상소서증가,청춘쌍기간균화기산유간균능강저Th1/Th2비례,기산유산간균효과경우.
Objective To observe and analyze the effect of B. Adolescentis and L. Acidophilus on the proportion of Th cell Th1/Th2 in peripheral blood of UC-mice in acute stage and recovery stage. Method 40 BABL/c mice were induced by 3% DSS water for 7 clays free drinking and then with distilled water for 10 days. They were randomly allocated in 4 groups: NS group, SASP group, BF0624 group and LT0637 group, also the fifth group-10 normal animals. The blood of mice were collected by removing their eyes at day 8 and day 18, and then the mononuclear cells were iso]atecl. The proportion of Th1/Th2 was analyze through flow eytometry,hy labeling the specific antibody ot Th cellular membrane with the CD4 antibody, and the cytoplastie antigen of Thl or Th2 with IIA antibody or IF'N-γ antibody. Result The proportion of Th1/Th2 in normal mouse was 0. 84 -0. 94,and it raised up in DSS-mice at both acute stage and recovery stage. It decreased unequally after 7 or 17 days'B, adoleseentis, L. Acidophilus and SASP treatment, but that of all three groups were lower than NS group (2.21±0. 83). Even the proportion got close to the normal animals after 17 days'L. Acidophilus -treated. Conclusion The proportion of Thl/Th2 increased at the acute stage and recovery stage of DSS-mice. Both B. Adoleseentis and L. Acidophilus had more effective than SASP on decreasing the proportion of Th1/Th2 at two stages,ospecially L. Acidophilus.