中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2008年
4期
204-207
,共4页
朱彬%王璞%刘子栋%吴春玲%高庆贞%王少梅%徐何
硃彬%王璞%劉子棟%吳春玲%高慶貞%王少梅%徐何
주빈%왕박%류자동%오춘령%고경정%왕소매%서하
NF-κB%内皮细胞%CD40配体%CD154配体%移植
NF-κB%內皮細胞%CD40配體%CD154配體%移植
NF-κB%내피세포%CD40배체%CD154배체%이식
NF-κB%Endothelial cell%CD40 ligand%CD154 ligand%Transplantation
目的 探讨血管内皮细胞(VEC)内核转录因子-κB(NF-κB)通路在CD40-CD154相互作用诱导VEC活化中的作用.方法 应用重组人肿瘤坏死因子α(rhTNF-α)和重组人γ干扰素(rhIFN-γ)刺激人主动脉VEC,通过流式细胞仪(FACS)检测CD40和粘附因子表达水平;通过表达CD154的细胞株D1.1和VEC共同培养后,观察VEC粘附因子的表达水平;通过抗CD154单克隆抗体阻断CD40-CD154间的反应;应用NF-κB阻断剂BAY11-7082观察阻断NF-κB通路后对CD40-CD154诱导VEC活化的影响.结果 未活化的VEC膜表达低水平的CD40、CD54和CD106,但不表达CD62E,经白细胞介素刺激后可上调这些蛋白分子的表达水平.D1.1细胞株和VEC共同培养后可诱导VEC活化并上调CD62E、CD54和CD106的表达水平;抗CD154抗体可阻断CD40-CD154间的反应所诱导的VEC活化;NF-κB阻断剂可阻断rhTNF-α诱导的VEC活化以及CD40-CD154间的反应所诱导的VEC活化.结论 VEC中CD40和T淋巴细胞中CD154之间的相互作用在诱导VEC活化过程中起重要作用;CD40-CD154相互作用并通过NF-κB通路诱导VEC活化;NF-κB通路阻断剂可抑制VEC活化.
目的 探討血管內皮細胞(VEC)內覈轉錄因子-κB(NF-κB)通路在CD40-CD154相互作用誘導VEC活化中的作用.方法 應用重組人腫瘤壞死因子α(rhTNF-α)和重組人γ榦擾素(rhIFN-γ)刺激人主動脈VEC,通過流式細胞儀(FACS)檢測CD40和粘附因子錶達水平;通過錶達CD154的細胞株D1.1和VEC共同培養後,觀察VEC粘附因子的錶達水平;通過抗CD154單剋隆抗體阻斷CD40-CD154間的反應;應用NF-κB阻斷劑BAY11-7082觀察阻斷NF-κB通路後對CD40-CD154誘導VEC活化的影響.結果 未活化的VEC膜錶達低水平的CD40、CD54和CD106,但不錶達CD62E,經白細胞介素刺激後可上調這些蛋白分子的錶達水平.D1.1細胞株和VEC共同培養後可誘導VEC活化併上調CD62E、CD54和CD106的錶達水平;抗CD154抗體可阻斷CD40-CD154間的反應所誘導的VEC活化;NF-κB阻斷劑可阻斷rhTNF-α誘導的VEC活化以及CD40-CD154間的反應所誘導的VEC活化.結論 VEC中CD40和T淋巴細胞中CD154之間的相互作用在誘導VEC活化過程中起重要作用;CD40-CD154相互作用併通過NF-κB通路誘導VEC活化;NF-κB通路阻斷劑可抑製VEC活化.
목적 탐토혈관내피세포(VEC)내핵전록인자-κB(NF-κB)통로재CD40-CD154상호작용유도VEC활화중적작용.방법 응용중조인종류배사인자α(rhTNF-α)화중조인γ간우소(rhIFN-γ)자격인주동맥VEC,통과류식세포의(FACS)검측CD40화점부인자표체수평;통과표체CD154적세포주D1.1화VEC공동배양후,관찰VEC점부인자적표체수평;통과항CD154단극륭항체조단CD40-CD154간적반응;응용NF-κB조단제BAY11-7082관찰조단NF-κB통로후대CD40-CD154유도VEC활화적영향.결과 미활화적VEC막표체저수평적CD40、CD54화CD106,단불표체CD62E,경백세포개소자격후가상조저사단백분자적표체수평.D1.1세포주화VEC공동배양후가유도VEC활화병상조CD62E、CD54화CD106적표체수평;항CD154항체가조단CD40-CD154간적반응소유도적VEC활화;NF-κB조단제가조단rhTNF-α유도적VEC활화이급CD40-CD154간적반응소유도적VEC활화.결론 VEC중CD40화T림파세포중CD154지간적상호작용재유도VEC활화과정중기중요작용;CD40-CD154상호작용병통과NF-κB통로유도VEC활화;NF-κB통로조단제가억제VEC활화.
Objective To determine human endothelial cells(VEC) activation induced by CD40-CD154 interaction and the role of NF-κB pathway in CD40-CD154 interaction. Methods VEC were isolated from aorta,and subcultures were stimulated by recombinant human TNF-α(rhTNF-α)or IFN-γ(rhIFN-γ). The expression of CD40 and adhesion molecules was detected by using flow cytometry. D1.1 cells expressing CD154 were co-incubated with VEC with or without CD154 specific monoclonal antibody, and the effects of CD40-CD154 interaction in induceding VEC activation were determined. In an additional study,BAY 11-7082,a NF-κB pathway inhibitor,was used to study whether CD40-CD154 interaction-induced VEC activation was NF-κB pathway-dependent. Results Resting VEC demonstrated weak expression of CD40,CD54 and CD106 but undetectable CD62E. VEC stimulated with rhTNF-α or rhINF-γ up-regulated the expression of CD40,CD62E,CD54 and CD106 respectively. VEC demonstrated up-regulation of CD62E, CD54 and CD106 after co-incubation with D1.1 cells,and VEC activation induced bv CD40-CD154 interaction was inhibited by CD154 specific monoclonal antibody. NF-κB could block the VEC activation induced bv rhTNF-α stimulation or by CD40-CD154 interaction. Conclusions The interaction between VEC-derived CD40 and CD154 expressing T cells plays a critical role in VEC activation. Inhibition of NF-κB during CD40-CD154 interaction prevents VEC activation.
