中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2008年
9期
899-904
,共6页
张海林%唐青%陶晓燕%王晓光%张云智%杨卫红%米竹青%王静林%章域震%冯云%郑维斌%鲁国勇%李胜国
張海林%唐青%陶曉燕%王曉光%張雲智%楊衛紅%米竹青%王靜林%章域震%馮雲%鄭維斌%魯國勇%李勝國
장해림%당청%도효연%왕효광%장운지%양위홍%미죽청%왕정림%장역진%풍운%정유빈%로국용%리성국
狂犬病病毒%基因%分子流行病学
狂犬病病毒%基因%分子流行病學
광견병병독%기인%분자류행병학
Rabies virus%Cene%Molecular epidemiology
目的 了解云南省保山市发生2例狂犬病患者的流行病学特点,根据病原分子生物学特征分析可能的传染来源.方法 采用狂犬病个案调查表进行流行病学调查,采集狂犬病死者脑组织标本,用直接免疫荧光试验(DFA)检测狂犬病病毒抗原,用RT-PCR法检测狂犬病病毒核酸,测定狂犬病病毒P、M和N基因全序列并根据其同源性比较及系统进化树进行分子流行病学分析.结果2006年7月保山市隆阳区发生1例人间狂犬病,2007年4月腾冲县发生1例狂犬病.2例患者犬伤暴露程度均为Ⅲ度.2例死亡病例均采集到脑组织(编号为CYN0601H和CYN0701H),经DFA和RT-PCR两种实验室方法检测确诊为狂犬病病毒阳性.CYN0601H和CYN0701H与国内外狂犬病病毒的P、M和N基因核苷酸和推导氨基酸序列同源性分析显示,CYN0601H和CYN0701H与泰国分离株的同源性最高.系统进化分析显示,2株病毒标本亲缘关系很近,同属于狂犬病病毒基因1型,且与泰国病毒株有较近的亲缘关系.结论从病原学和病毒分子水平证实云南省保山市2例患者均为狂犬病,保山市狂犬病病毒流行株可能为境外传人,应加强云南省边境地区狂犬病防制工作.
目的 瞭解雲南省保山市髮生2例狂犬病患者的流行病學特點,根據病原分子生物學特徵分析可能的傳染來源.方法 採用狂犬病箇案調查錶進行流行病學調查,採集狂犬病死者腦組織標本,用直接免疫熒光試驗(DFA)檢測狂犬病病毒抗原,用RT-PCR法檢測狂犬病病毒覈痠,測定狂犬病病毒P、M和N基因全序列併根據其同源性比較及繫統進化樹進行分子流行病學分析.結果2006年7月保山市隆暘區髮生1例人間狂犬病,2007年4月騰遲縣髮生1例狂犬病.2例患者犬傷暴露程度均為Ⅲ度.2例死亡病例均採集到腦組織(編號為CYN0601H和CYN0701H),經DFA和RT-PCR兩種實驗室方法檢測確診為狂犬病病毒暘性.CYN0601H和CYN0701H與國內外狂犬病病毒的P、M和N基因覈苷痠和推導氨基痠序列同源性分析顯示,CYN0601H和CYN0701H與泰國分離株的同源性最高.繫統進化分析顯示,2株病毒標本親緣關繫很近,同屬于狂犬病病毒基因1型,且與泰國病毒株有較近的親緣關繫.結論從病原學和病毒分子水平證實雲南省保山市2例患者均為狂犬病,保山市狂犬病病毒流行株可能為境外傳人,應加彊雲南省邊境地區狂犬病防製工作.
목적 료해운남성보산시발생2례광견병환자적류행병학특점,근거병원분자생물학특정분석가능적전염래원.방법 채용광견병개안조사표진행류행병학조사,채집광견병사자뇌조직표본,용직접면역형광시험(DFA)검측광견병병독항원,용RT-PCR법검측광견병병독핵산,측정광견병병독P、M화N기인전서렬병근거기동원성비교급계통진화수진행분자류행병학분석.결과2006년7월보산시륭양구발생1례인간광견병,2007년4월등충현발생1례광견병.2례환자견상폭로정도균위Ⅲ도.2례사망병례균채집도뇌조직(편호위CYN0601H화CYN0701H),경DFA화RT-PCR량충실험실방법검측학진위광견병병독양성.CYN0601H화CYN0701H여국내외광견병병독적P、M화N기인핵감산화추도안기산서렬동원성분석현시,CYN0601H화CYN0701H여태국분리주적동원성최고.계통진화분석현시,2주병독표본친연관계흔근,동속우광견병병독기인1형,차여태국병독주유교근적친연관계.결론종병원학화병독분자수평증실운남성보산시2례환자균위광견병,보산시광견병병독류행주가능위경외전인,응가강운남성변경지구광견병방제공작.
Objective To understand the epidemiological features of two rabies cases in Baoshan city year 2006 and 2007 and to analyze its source of infection.Methods Questionnaires were used to do the epidemiologieal survey on each of the rabies cases.Brain timue samples of rabies patients were collet to detect the rabies virus by direct immunofluoreseence assay(DFA)and RT-PCR assay.Homology and phylogenetic tree were analyzed.based on the whole nucleotide and deduced amino acid sequence of P,M and N gene of rabies virus followed by molecular epidemiological analysis.Results In July 2006,one human rabies case was identified in Longyang district,and another one in Tengchong county in Baoshan city in 2007.The degrees of exposure of these two patients was all at degreeⅢ.Two brain tissue samples among the dead patients(No.CYN0601H and CYN0701H)were confirmed positive by both DFA and RTPCR assay.The homology analysis of P,M and N gene sequences among CYN0601H,CYN0701H and other rabias strains isolated from other provinces and other counties.showed that the samples in Baoshan city shared the highest homology with the strains in Thailand.Phylogenetic analysis indicated that the two samples were very dose and all belonged to genetype 1 Lyssavirus,with the closest relationship between samples in Baoshan city and strains in Thailand.Conclusion It Was confirmed on the virus molecular level that the two patients in Baoshan city were both suffered from rabies.The prevalent strains in Baoshan city WaS probably imported from foreign country,suggesting that prevention and control measures on rabies virus in the boarder areas of Yunnan should be strengthened.
