CAJ | 학술논문

目的探讨年龄相关性白内障患者与未患该病的一般人血细胞以及培养的晶状体上皮SRA01/04细胞的DNA修复酶XPD-751、XRCCl-399以及羟自由基水平的差异.方法实验研究.2008年3月至2009年10月,自郑州人民医院30例白内障患者手术切除之晶状体上皮细胞和30例同龄未患该病的一般人的血细胞以及培养的SRA01/04细胞分别提取基因组DNA进行实验研究,应用PCR-限制性内切酶长度多态性技术检测其DNA修复酶XPD-751 /XRCC1-399的多态性,并以SRA01/04细胞和白内障患者的周血细胞分别作为对照,同时进行羟自由基检测.所得数据组间比较应用单因素方差分析.结果未患白内障的一般人及SRA01/04细胞均呈现XRCC1-Gln399Gln和XPD-Lys751Lys.白内障患者晶状体上皮细胞及其周血细胞皆呈现XPD-Lys751 Gln多态性和XRCC1-Gln399Gln型,后者与前者之间均未见差异.白内障患者晶状体上皮细胞和外周血细胞的羟自由基分别为(0.204±0.7)和(0.188±0.07),高于未患白内障的一般人(t=211.20,P＜ 0.05).结论年龄相关性白内障患者晶状体上皮细胞DNA修复酶呈现XDP-Lys751Gln多态性且羟自由基水平较高,可能与晶状体白内障的罹病风险有关.
목적 탐토년령상관성백내장환자여미환해병적일반인혈세포이급배양적정상체상피SRA01/04세포적DNA수복매XPD-751、XRCCl-399이급간자유기수평적차이.방법 실험연구.2008년3월지2009년10월,자정주인민의원30례백내장환자수술절제지정상체상피세포화30례동령미환해병적일반인적혈세포이급배양적SRA01/04세포분별제취기인조DNA진행실험연구,응용PCR-한제성내절매장도다태성기술검측기DNA수복매XPD-751 /XRCC1-399적다태성,병이SRA01/04세포화백내장환자적주혈세포분별작위대조,동시진행간자유기검측.소득수거조간비교응용단인소방차분석.결과 미환백내장적일반인급SRA01/04세포균정현XRCC1-Gln399Gln화XPD-Lys751Lys.백내장환자정상체상피세포급기주혈세포개정현XPD-Lys751 Gln다태성화XRCC1-Gln399Gln형,후자여전자지간균미견차이.백내장환자정상체상피세포화외주혈세포적간자유기분별위(0.204±0.7)화(0.188±0.07),고우미환백내장적일반인(t=211.20,P＜ 0.05).결론 년령상관성백내장환자정상체상피세포DNA수복매정현XDP-Lys751Gln다태성차간자유기수평교고,가능여정상체백내장적리병풍험유관.
Objective To explore the genotype characteristics of DNA repair enzyme XPD-751 and XRCC1-399 in the lens epithelium of cataract patients,and difference in free radical level.Methods The genome DNA was extracted from respective lens epithelium of 30 cataract patients,their peripheral blood and 12 normal control's peripheral blood by using a Genomic DNA kit,and the polymorphisms of DNA repair enzymes XPD-751 and XRCC1-399 were examined by PCR-RFLP technique. Meanwhile,that of the cultured normal human lens epithelial SRA01/04 cells was carried out same way as reference control.Besides,the free radical level was detected by related kit. Results The XRCC1- Gln399Gln or XPDLys751Lys genotype was found in the normal control group and cultured human SRA01/04 cells,while the polymorphism of XPD-Lys751Gln could be found in both lens epithelium and blood cells of the cataract patient group. However,there was no difference in XRCC1-399 phenotype between the patient and the normal control groups.There is difference in free radical level between the cataract(0.204 ±0.07;0.188 ±0.07 ) and healthy subjects ( t =211.20,P ＜ 0.05 ).Conclusion The polymorphism of XPD-Lys751 Gln can be found in the lens epithelium of cataract patient group,suggesting that there may be association among the XPD-751 polymorphism with higher free radical level in the age-related cataract disease.