CAJ | 학술논문

目的观察不同剂量抗血管内皮生长因子单克隆抗体Bevacizumab兔眼玻璃体腔注射的视网膜毒性作用. 方法 16只新西兰无色素兔的32只眼随机分为药物注射组和对照组,药物注射组又根据玻璃体腔注射药物剂量不同分为A、B、C组,玻璃体腔注射Bevacizumab剂量分别为0.05、0.10、0.25ml,分别含Bevacizumab 1.25、2.50、6.25 mg.对照组玻璃体腔注射0.9%生理盐水0.10 ml.注药后1、2、4周行视网膜电图(ERG)检查.另外,在兔眼玻璃体腔注射Bevacizumab后1、2、4周,每组各摘除2只兔眼,行视网膜组织形态及超微结构的光学显微镜和透射电子显微镜观察. 结果兔眼玻璃体腔注射Bevacizumab后1、2、4周,兔眼ERG各项反应波形均正常,振幅均未出现异常改变(P＞0.05).光学显微镜下观察,药物注射组和对照组视网膜各层组织形态在各时间点均未见异常.透射电子显微镜观察,A、B组与对照组无明显差异;C组视网膜光感受器细胞出现部分线粒体损伤,发生肿胀和积水变,4周时病变无缓解.结论单次兔眼玻璃体腔注射Bevacizumab 1.25 mg或2.50 mg是安全的.
목적 관찰불동제량항혈관내피생장인자단극륭항체Bevacizumab토안파리체강주사적시망막독성작용. 방법 16지신서란무색소토적32지안수궤분위약물주사조화대조조,약물주사조우근거파리체강주사약물제량불동분위A、B、C조,파리체강주사Bevacizumab제량분별위0.05、0.10、0.25ml,분별함Bevacizumab 1.25、2.50、6.25 mg.대조조파리체강주사0.9%생리염수0.10 ml.주약후1、2、4주행시망막전도(ERG)검사.령외,재토안파리체강주사Bevacizumab후1、2、4주,매조각적제2지토안,행시망막조직형태급초미결구적광학현미경화투사전자현미경관찰. 결과토안파리체강주사Bevacizumab후1、2、4주,토안ERG각항반응파형균정상,진폭균미출현이상개변(P＞0.05).광학현미경하관찰,약물주사조화대조조시망막각층조직형태재각시간점균미견이상.투사전자현미경관찰,A、B조여대조조무명현차이;C조시망막광감수기세포출현부분선립체손상,발생종창화적수변,4주시병변무완해.결론 단차토안파리체강주사Bevacizumab 1.25 mg혹2.50 mg시안전적.
Objective To observe the retinal toxicity of intravitreal injection of Bevacizumab (Avastin)in albino rabbit eyes at different doses. Methods Sixteen New Zealand albino rabbits,thirtytwo eyes were divided into four groups at random.Three groups were prepared for Avastin experiment,named A,B,C.Each group received intravitreal injection of Avastin at dose 1.25 mg/0.05 ml,2.5 mg/0.1 ml and 6.25 mg/0.25 m1 respectively.The other group named D served as a control,and accepted intravitreal injection of 0.9%normal saline 0.1 ml.Then test it by electroretinagram(ERG)after 1,2 and 4 weeks.In addition,each group was removing two rabbit's eyes to observe the retinal morphology and ultrastructure by light microscope and transmission electron microscopy after intravitreal injection avastin 1,2 and 4 weeks. Results The ERG pattern and amplitude of each group were normal after intravitreal injection Avastin 1,2 and 4 weeks.(P＞0.05)Between study and control groups,there was no significant difference in retinal morphology which was observed by light microscope at any stage of the study.By electron microscopic observation,retinal ultramicrostructure was no evident retinal toxicity being tested both at group A and B(1.25 mg/0.05 ml and 2.5 mg/0.1 ml).But at group C (6.25 mg/0.25 ml),significant mitochondrial swelling and hydropic changes were seen in the inner segments of photoreceptors.And there was no improvement of the pathological changes in four weeks.Conclusion It is safe that intravitreal injection of Avastin in rabbit's eyes at dose 1.25 mg or 2.5 mg atsingle time.