CAJ | 학술논문

目的抗原冲击的树突状细胞(DC)与肿瘤周围皮下给予白细胞介素-2(IL-2)分别治疗大肠癌,目的在于比较二者抗肿瘤作用,为其应用于临床提供实验依据.方法将荷C26结肠癌的BALB/C(H-2d)小鼠随机分为对照组、IL-2治疗组、抗原冲击的DC治疗组.抗原冲击的DC治疗组在荷瘤后第1、8、15、22天于右侧腹股沟皮下注射抗原冲击的DC;IL-2治疗组在荷瘤后第1～5、8～12、15～19、22～26天于肿瘤周围皮下注射IL-2,对照组在相同的部位注射PBS液.观察荷瘤小鼠的肿瘤体积、生存期和存活率.结果两种治疗方案均能明显抑制肿瘤生长,延长小鼠的生存期(P<0.05);但抗原冲击的DC组较IL-2组更能延长荷瘤小鼠的生存期(P<0.05).结论抗原冲击的DC组较IL-2组明显延长荷瘤小鼠的存活时间而达到更明显的抗肿瘤作用.
목적 항원충격적수돌상세포(DC)여종류주위피하급여백세포개소-2(IL-2)분별치료대장암,목적 재우비교이자항종류작용,위기응용우림상제공실험의거.방법 장하C26결장암적BALB/C(H-2d)소서수궤분위대조조、IL-2치료조、항원충격적DC치료조.항원충격적DC치료조재하류후제1、8、15、22천우우측복고구피하주사항원충격적DC;IL-2치료조재하류후제1～5、8～12、15～19、22～26천우종류주위피하주사IL-2,대조조재상동적부위주사PBS액.관찰하류소서적종류체적、생존기화존활솔.결과 량충치료방안균능명현억제종류생장,연장소서적생존기(P<0.05);단항원충격적DC조교IL-2조경능연장하류소서적생존기(P<0.05).결론 항원충격적DC조교IL-2조명현연장하류소서적존활시간이체도경명현적항종류작용.
Objective Tumor lysate-pulsed DC and systemic administration of interleukin-2(IL-2) was used in our experiment to therapy mice colon tumor model in order to investigate its anti-tumor mechanisms and provide preclinical rationale experimental theory. Methods C26 tumor-bearing BALB/C(H-2d) mice were randomized over control groups and IL-2 groups, C26 tumor lysate-pulsed DC groups. C26 tumor lysate-pulsed DC groups were injected s.c with tumor lysate-pulsed DC at days 1,8,15,22. IL-2 groups were injected peritumorally with IL-2 at days 1-5,8-12,15-19,22-26.Control groups were injected peritumorally with PAS.The tumor volume, the survival time and the survival rate was recorded. Results The two therapeutic methods inhibited significantly the tumor growth and enhanced survival(P<0.05). The therapeutic effect of C26 tumor lysate-pulsed DC treatments is much better than the effect of IL-2 treatments. Tumor lysate-pulsed DC groups enhanced survival in comparison with IL-2 groups(P<0.05). Conclusions The therapeutic effect of C26 tumor lysate-pulsed DC treatments was much better than the effect of IL-2 treatments in survival.