CAJ | 학술논문

目的探讨基因芯片在诊断β地中海贫血中的价值.方法对经筛查初诊的32例β地贫进行基因芯片检测并与斑点杂交法进行比较分析.结果 32例β地贫中,基因芯片技术检出32例,斑点杂交法检出30例,两者差别无统计意义(P＞0.05).结论基因芯片技术与斑点杂交法在β地贫的诊断上可以互相代替;基因芯片技术具有快速、高效、自动化等特点且简便、经济、省时省力,较斑点杂交法优越,宜提倡推广应用.
목적탐토기인심편재진단β지중해빈혈중적개치.방법대경사사초진적32례β지빈진행기인심편검측병여반점잡교법진행비교분석.결과 32례β지빈중,기인심편기술검출32례,반점잡교법검출30례,량자차별무통계의의(P＞0.05).결론기인심편기술여반점잡교법재β지빈적진단상가이호상대체;기인심편기술구유쾌속、고효、자동화등특점차간편、경제、성시성력,교반점잡교법우월,의제창추엄응용.
Objective:Diagnosis of beta-thalassemia was investigated by using gene chip techniques.Methods:32 cases of beta-thalassemia screened by routine methods were determined by using gene chip techniques and spot hybridization.Results:All of 32 patients suffering from beta-thalassemia were diagnosed by using gene chip techniques,in which 30 cases were determined by spot hybridization.There was no statistical difference between the two methods.Conclusion:Gene chip techniques were simple and display much more advantages than spot hybridization such as taking short time,presenting high efficiency and costing less,etc.therefore,spot hybridization can be replaced by gene chip techniques.