CAJ | 학술논문

目的研究醋酸艾塞那肽(exendin-4)在大鼠体内的组织分布.方法 Iodo-GenTM法制备[125I]exendin-4, 大鼠皮下注射[125I]exendin-4后, 分别测定血浆或组织中的总放射性含量和酸沉淀放射性含量.结果 [125I]exendin-4的酸沉淀放射性分布从高到低的顺序为肾脏＞肺＞膀胱＞胰腺＞肠＞血浆＞肾上腺＞空肠＞淋巴结＞肝＞脾＞心脏＞骨髓＞胸腺＞睾丸＞脑＞肌肉＞脂肪.结论 [125I]exendin-4的分布快速而广泛,其中以肾脏中最高,而在脑组织只发现微量的[125I]exendin-4.
목적 연구작산애새나태(exendin-4)재대서체내적조직분포.방법 Iodo-GenTM법제비[125I]exendin-4, 대서피하주사[125I]exendin-4후, 분별측정혈장혹조직중적총방사성함량화산침정방사성함량.결과 [125I]exendin-4적산침정방사성분포종고도저적순서위신장＞폐＞방광＞이선＞장＞혈장＞신상선＞공장＞림파결＞간＞비＞심장＞골수＞흉선＞고환＞뇌＞기육＞지방.결론 [125I]exendin-4적분포쾌속이엄범,기중이신장중최고,이재뇌조직지발현미량적[125I]exendin-4.
AIM To investigate the tissue distribution of exendin-4 after administration in healthy rats. METHODS Exendin-4 was radioiodinated by the Iodo-GenTMmethod. Tissue distribution of [125I]exendin-4 was investigated after sc administration of [125I]exendin-4 at 3 μg·kg-1 in rats. Both total radioactivity and trichloroacetic acid (TCA) precipitated radioactivity were used to calculate the levels of [125I]exendin-4 in rats plasma and tissue samples after sc administration. RESULTS The tissue distribution of [125I]exendin-4 after sc injection showed substantial disposition in kidneys, lungs, bladder and pancreas. The rank order of normalized tissue distribution was kidneys＞lungs＞bladder＞pancreas＞intestine＞plasma＞adrenals>jejunum＞lymph＞liver＞spleen＞heart＞marrow＞thymus＞testicles＞brain＞muscle＞adipose. CONCLUSION [125I]Exendin-4 underwent a rapid and wide distribution in the tissues throughout the whole body within the time course examined. TCA precipitated radioactivity in kidneys was the highest, however, only trace amounts of [125I]exendin-4 was detected in the brain.