CAJ | 학술논문

目的采用体外培养方式，探讨日本血吸虫未成熟卵可溶性抗原（SIEA）免疫血清的抗卵胚胎发育作用。方法从日本血吸虫小鼠肝脏无菌分离提取虫卵，转入RPMI-1640培养基中，对照组与试验组分别加入正常鼠血清和SIEA免疫血清。试验1：培养虫卵30d;试验2：培养虫卵14d。观察统计各期虫卵比较。结果在试验1中，SIEA免疫血清组成熟卵比例与对照组比较，下降22.5％，初产卵、胚胎卵和死亡卵比例均高于对照组。在试验2中，SIEA免疫血清组成熟卵比例下降14.7％，胚胎卵比例上升24.4％。结论体外培养系统中SIEA免疫血清具有抗日本血吸虫卵胚胎发育的作用。
목적 채용체외배양방식，탐토일본혈흡충미성숙란가용성항원（SIEA）면역혈청적항란배태발육작용。방법 종일본혈흡충소서간장무균분리제취충란，전입RPMI-1640배양기중，대조조여시험조분별가입정상서혈청화SIEA면역혈청。시험1：배양충란30d;시험2：배양충란14d。관찰통계각기충란비교。결과 재시험1중，SIEA면역혈청조성숙란비례여대조조비교，하강22.5％，초산란、배태란화사망란비례균고우대조조。재시험2중，SIEA면역혈청조성숙란비례하강14.7％，배태란비례상승24.4％。결론 체외배양계통중SIEA면역혈청구유항일본혈흡충란배태발육적작용。
Objective Study the anti-embryonation of Schistosoma japonicumeggs by soluble immature egg antigen (SIEA) immunized serum in vitro.Methods Eggs were collected from livers of schistosomiasis mice,transfered into medium RPMI-1640,adding normal mice serum or anti-SIEA serum in control group and immune group,respectively.Eggs were cultivated for 30 days in test 1,cultivated for 14 days in test 2.Results In test 1,by comparing with control group,the percentage of mature eggs in immune group of anti-SIEA serum was obviously decreased by 22.5%.The percentage of primary eggs,embryonic eggs and dead eggs were all higher in immune group than those in control group.In test 2,percentage of mature eggs dropped 14.7%,but percentage of embryonic eggs increased by 24.4% in immune group.Conclusions The result indicated that anti-SIEA serum can inhibit the embryonic development of Schistosoma japonicum immature eggs in vitro.