CAJ | 학술논문

采用水培方法,研究不同浓度Se~(4+)对蚕豆胚根抗氧化酶系统的影响.结果表明,随着Se~(4+)浓度的升高,SOD活性一直升高,而CAT活性下降;当Se~(4+)浓度低于0.01 μmol/L时POD活性随着浓度升高而下降,Se~(4+)浓度高于0.01 μmol/L时POD活性开始升高;Se~(4+)浓度低于0.005 μmol/L时MDA含量随浓度升高而降低,Se~(4+)浓度高于0.01 μmol/L时MDA含量开始升高.对POD同功酶的聚丙烯酰胺凝胶电泳结果分析表明,不同浓度的Se~(4+)处理蚕豆根POD同功酶没有特异性条带的表达,但活性强弱不同,有的带已经消失了.
채용수배방법,연구불동농도Se~(4+)대잠두배근항양화매계통적영향.결과표명,수착Se~(4+)농도적승고,SOD활성일직승고,이CAT활성하강;당Se~(4+)농도저우0.01 μmol/L시POD활성수착농도승고이하강,Se~(4+)농도고우0.01 μmol/L시POD활성개시승고;Se~(4+)농도저우0.005 μmol/L시MDA함량수농도승고이강저,Se~(4+)농도고우0.01 μmol/L시MDA함량개시승고.대POD동공매적취병희선알응효전영결과분석표명,불동농도적Se~(4+)처리잠두근POD동공매몰유특이성조대적표체,단활성강약불동,유적대이경소실료.
The authors investigated antioxidant enzyme system in the radicle of Vicia Faba treated with different concentrations of Se~(4+).The results indicated that SOD activity increased and CAT acvtivity decreased with the increase of the concentration of Se~(4+);if the concentration was lower than 0.01 μmol/L POD activity decreased with the increase of the concentration;when the concentration was lower than 0.005 μmol/L Se~(4+) the content of MDA decreased as concentration increased.By polyacrylancide gel electrophoresis(PAGE),different concentrations of Se~(4+) did not induce the expression of special strips for POD isoenzymes,but the activities were different from each other,some strips disappeared.