中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2012年
3期
195-198
,共4页
胡传义%易成%蒋林涛%张科%张满%刘昌荣
鬍傳義%易成%蔣林濤%張科%張滿%劉昌榮
호전의%역성%장림도%장과%장만%류창영
肝细胞生长因子%前列腺肿瘤%新生血管化,病理性
肝細胞生長因子%前列腺腫瘤%新生血管化,病理性
간세포생장인자%전렬선종류%신생혈관화,병이성
Hepatocyte growth factor%Prostatic neoplasms%Neovascularization,pathologic
目的 探讨肝细胞生长因子激活物抑制剂1(HAI-1)在前列腺癌中的表达及其与肿瘤血管生成的关系.方法 应用半定量RT-PCR法检测HAI-1基因在48例前列腺癌组织和20例前列腺增生组织中的表达水平；采用免疫组化SP法测定上述组织CD34的表达,并进行微血管密度(MVD)计数.分析HAI-1基因表达与前列腺癌临床病理指标及MVD的关系.结果 20例前列腺增生组织中,HAI-1的阳性率为85.00％,而48例前列腺癌组织的阳性率为52.08％,差异有统计学意义(P＜0.05).前列腺癌A+B期HAI-1的阳性率(71.43％)显著高于C+D期(37.04％,P＜0.05).前列腺癌组织中HAI-1基因的表达与MVD计数之间呈负相关(r=-0.647,P＜0.05).结论 HAI -1基因的表达缺失可能与前列腺癌的发生发展、浸润转移存在一定的关系.
目的 探討肝細胞生長因子激活物抑製劑1(HAI-1)在前列腺癌中的錶達及其與腫瘤血管生成的關繫.方法 應用半定量RT-PCR法檢測HAI-1基因在48例前列腺癌組織和20例前列腺增生組織中的錶達水平；採用免疫組化SP法測定上述組織CD34的錶達,併進行微血管密度(MVD)計數.分析HAI-1基因錶達與前列腺癌臨床病理指標及MVD的關繫.結果 20例前列腺增生組織中,HAI-1的暘性率為85.00％,而48例前列腺癌組織的暘性率為52.08％,差異有統計學意義(P＜0.05).前列腺癌A+B期HAI-1的暘性率(71.43％)顯著高于C+D期(37.04％,P＜0.05).前列腺癌組織中HAI-1基因的錶達與MVD計數之間呈負相關(r=-0.647,P＜0.05).結論 HAI -1基因的錶達缺失可能與前列腺癌的髮生髮展、浸潤轉移存在一定的關繫.
목적 탐토간세포생장인자격활물억제제1(HAI-1)재전렬선암중적표체급기여종류혈관생성적관계.방법 응용반정량RT-PCR법검측HAI-1기인재48례전렬선암조직화20례전렬선증생조직중적표체수평；채용면역조화SP법측정상술조직CD34적표체,병진행미혈관밀도(MVD)계수.분석HAI-1기인표체여전렬선암림상병리지표급MVD적관계.결과 20례전렬선증생조직중,HAI-1적양성솔위85.00％,이48례전렬선암조직적양성솔위52.08％,차이유통계학의의(P＜0.05).전렬선암A+B기HAI-1적양성솔(71.43％)현저고우C+D기(37.04％,P＜0.05).전렬선암조직중HAI-1기인적표체여MVD계수지간정부상관(r=-0.647,P＜0.05).결론 HAI -1기인적표체결실가능여전렬선암적발생발전、침윤전이존재일정적관계.
Objective To investigate the expression of hepatocyte growth factor activator inhibitor (HAI) - 1 gene in the tissues of prostatic carcinoma and its correlation with tumor angiogenesis.Methods The expression of HAI-1 gene was detected in 48 cases of prostatic carcinoma and 20 cases of prostatic hyperplasia tissues by semi-quantitative RT-PCR,and the expression of CD34 in the tissues mentioned above was detected by immunohistochemistry SP method.Thereafter,the microvessel density (MVD) was calculated in order to analyze the correlation of HAI-1 gene expression and the clinical pathological indicators of prostatic carcinoma.Results Thc positive expression rate of HAI- 1 gene was 85.00％ in 20 cases of prostatic hyperplasia tissues and 52.08％ in 48 cases of prostatic csarcinoma tissues (P＜0.05).The positive expression rate of HAI-1 gene in A + B stage of prostatic carcinoma (71.43％) was significantly higher than that in C +D stage (37.04％) (P＜0.05).The positive expression rate of HAI-1 gene in poorly differentiated group was obviously lower than that in moderately and highly differentiated groups (P＜0.05).The MVD in prostatic carcinoma tissues was significantly higher than that in prostatic hyperplasia tissues (P＜0.01).The expression of HAI- 1 gene was negatively correlated with MVD in prostatic carcinoma tissues (r=-0.647,P＜ 0.05).Conclusion The deficiency of HAI-1 expression may have a certain correlation with the development and infiltration metastasis of prostatic carcinoma.
