CAJ | 학술논문

目的探讨肝细胞癌的预后因素及磷脂酰肌醇蛋白聚糖3( GPC3)、肝细胞抗原(HEP)、甲胎蛋白(AFP)、CD34及CD10蛋白表达的预后意义.方法对375例肝细胞癌患者进行临床病理及预后分析,对其中80例肝细胞癌患者的石蜡标本以EliVision免疫组织化学染色法进行上述抗体染色,分析抗体表达与病理特征的关系,并进行生存分析.结果肿瘤数目(P=0.000)、肿瘤大小(P=0.025)、分化程度(P =0.001)及血管浸润(P=0.000)均为与预后有关的因素.GPC3( 66/80,82.5％)、HEP( 64/80,80.0％)、AFP(38/80,47.5％)及CD10( 28/80,35.0％)表达与分化程度明显相关,其P值分别为0.002、0.021、0.014、0.002.GPC3表达还与肿瘤单发与多发、有无卫星灶相关,P =0.028.AFP、CD10的表达与脉管有无癌栓密切相关,P值分别为0.000、0.010.KaplanMeier法生存分析显示HEP低表达及AFP高表达者预后差.结论肿瘤大小、数目、分化程度及血管浸润为肝细胞癌预后相关因素；HEP及AFP表达情况有明显的预后意义.
목적 탐토간세포암적예후인소급린지선기순단백취당3( GPC3)、간세포항원(HEP)、갑태단백(AFP)、CD34급CD10단백표체적예후의의.방법 대375례간세포암환자진행림상병리급예후분석,대기중80례간세포암환자적석사표본이EliVision면역조직화학염색법진행상술항체염색,분석항체표체여병리특정적관계,병진행생존분석.결과 종류수목(P=0.000)、종류대소(P=0.025)、분화정도(P =0.001)급혈관침윤(P=0.000)균위여예후유관적인소.GPC3( 66/80,82.5％)、HEP( 64/80,80.0％)、AFP(38/80,47.5％)급CD10( 28/80,35.0％)표체여분화정도명현상관,기P치분별위0.002、0.021、0.014、0.002.GPC3표체환여종류단발여다발、유무위성조상관,P =0.028.AFP、CD10적표체여맥관유무암전밀절상관,P치분별위0.000、0.010.KaplanMeier법생존분석현시HEP저표체급AFP고표체자예후차.결론 종류대소、수목、분화정도급혈관침윤위간세포암예후상관인소；HEP급AFP표체정황유명현적예후의의.
Objective To explore prognostic factors and the expression of glypican-3,hepatocyte antigen(HEP),alpha-fetoprotein(AFP),CD34 and CD10 in hepatocellular carcinoma (HCC) and their prognostic value.Methods Clinicopathologic data were analyzed in 375 cases of HCC,in which 80 cases with follow-up were examined by immunohistochemical staining to detect the expression of glypican-3,HEP,AFP,CD34 and CD10 proteins. The relationship between the proteins expression and clinicopathologic features was also evaluated. Results Tumor number ( P =0.000 ),tumor size ( P =0.025 ),tumor differentiation (P =0.001)and vessel invasion (P =0.000 )were closely related to prognosis of HCC patients; the expression of glypican-3 ( 66/80,82.5％ ;P =0.002),HEP(64/80,80.0％ ;P =0.021),AFP (38/80,47.5％ ;P =0.014 ) and CD10 ( 28/80,35.0％ ; P =0.002 ) was significantly related to tumor differentiation; that of glypican-3 was significantly correlated with tumor number and presence of satellite nodules(P =0.028)and that of AFP and CD10 was significantly correlated with portal vein thrombi (P=0.000,P =0.010). On Kaplan-Meier regression analysis,both low expression of HEP and high expression of AFP were closely related to poor prognosis.Conclusions Tumor number,size,differentiation and vessel invasion were important factors affecting the prognosis of patients with HCC.HEP and AFP have prognostic significance in HCC.