CAJ | 학술논문

目的探讨治疗性高碳酸血症对大鼠移植肺肺泡Ⅱ型上皮细胞(ATⅡ)的影响.方法肺移植成功后的成年雄性Wistar大鼠28只,周龄6周,体重180～220 g,采用随机数字表法,将大鼠随机分为2组(n=14):对照组(C组)和治疗性高碳酸血症组(T组).再灌注期间C组吸入50%O250%N2;T组吸入O2、N2和CO2混合气体,调整混合气体的浓度,维持吸入氧浓度50%,动脉血二氧化碳分压80～100 min Hg,再灌注90 min时取移植肺肺静脉血样行血气分析,计算氧合指数;采用酶消化法分离、纯化ATⅡ,鉴定并观察超微结构;流式细胞术检测ATⅡ凋亡情况.结果与C组比较,T组氧合指数增高,ATⅡ凋亡率降低(P＜0.05).T组ATⅡ超微结构损伤程度轻于C组.结论治疗性高碳酸血症可减少大鼠移植肺ATⅡ凋亡,从而改善肺功能.
목적 탐토치료성고탄산혈증대대서이식폐폐포Ⅱ형상피세포(ATⅡ)적영향.방법폐이식성공후적성년웅성Wistar대서28지,주령6주,체중180～220 g,채용수궤수자표법,장대서수궤분위2조(n=14):대조조(C조)화치료성고탄산혈증조(T조).재관주기간C조흡입50%O250%N2;T조흡입O2、N2화CO2혼합기체,조정혼합기체적농도,유지흡입양농도50%,동맥혈이양화탄분압80～100 min Hg,재관주90 min시취이식폐폐정맥혈양행혈기분석,계산양합지수;채용매소화법분리、순화ATⅡ,감정병관찰초미결구;류식세포술검측ATⅡ조망정황.결과 여C조비교,T조양합지수증고,ATⅡ조망솔강저(P＜0.05).T조ATⅡ초미결구손상정도경우C조.결론 치료성고탄산혈증가감소대서이식폐ATⅡ조망,종이개선폐공능.
Objective To investigate the effects of therapeutic hypercapnia on type Ⅱ alveolar cells (ATⅡ ) in the transplanted lung in rats.Methods Twenty-eight pathogen free adult male Wistar rats weighing 180-220 g were randomly divided into 2 groups (n= 14 each) : control group (group C) and therapeutic hypercapnia group (group T). The animals were anesthetized with intraperitoneal 3% pentobarbital 30 mg/kg, tracheostomized and mechanically ventilated with 50% O2-50% N2 (VT 10 ml/kg, RR 60 bpm, I∶ E1∶1). Left lung transplantation was performed. In group T starting from the beginning of reperiusion of the transplanted lung, the animals were ventilated with a mixture of 50% O2-N2 and C02(in appropriate concentrations) to keep PaCO2 between 80-100 mm Hg. After 90 min of reperfusion of the transplanted lung, blood samples were collected from pulmonary vein of the transplanted lung and blood gas analysis was performed. Oxygenation index was calculated.AT II cells were isolated from the transplanted lung and purified and examined with electronic microscope. The apoptosis rate of AT Ⅱ cells was detected by flow cytometry. Results Oxygenation index was significantly higher, the apoptotic rate of ATⅡ cells lower, the damage to ATⅡ cells was less in group T than in group C.Conclusion Therapeutic hypercapnia can protect the AT Ⅱ cells in the transplanted lung and improve the function of the trans planted lung.