CAJ | 학술논문

目的评价丙酮酸乙酯预先给药对原位肝移植术大鼠肝损伤的影响.方法成年健康雄性SD大鼠40只,体重220～ 250 g,作为肝移植术的供体和受体.采用随机数字表法,将受体大鼠随机分为3组(n=8):假手术组(S组)、肝移植组(LT组)和丙酮酸乙酯组(EP组).S组仅行单纯开关腹手术,LT组和EP组分别采用二袖套法建立大鼠原位肝移植模型,EP组于切皮前1h时尾静脉注射丙酮酸乙酯40 mg/kg.于新肝期2h时采集静脉血样,检测血清ALT和AST的活性；取肝中叶组织检测MDA含量和SOD活性.结果与S组比较,LT组和EP组血清ALT、AST的活性和肝组织MDA含量升高,SOD活性降低(P＜0.05或0.01)；与LT组比较,EP组血清ALT、AST的活性和肝组织MDA含量降低,SOD活性升高(P＜0.05或0.01).结论丙酮酸乙酯预先给药可减轻原位肝移植术大鼠肝损伤.
목적 평개병동산을지예선급약대원위간이식술대서간손상적영향.방법 성년건강웅성SD대서40지,체중220～ 250 g,작위간이식술적공체화수체.채용수궤수자표법,장수체대서수궤분위3조(n=8):가수술조(S조)、간이식조(LT조)화병동산을지조(EP조).S조부행단순개관복수술,LT조화EP조분별채용이수투법건립대서원위간이식모형,EP조우절피전1h시미정맥주사병동산을지40 mg/kg.우신간기2h시채집정맥혈양,검측혈청ALT화AST적활성；취간중협조직검측MDA함량화SOD활성.결과 여S조비교,LT조화EP조혈청ALT、AST적활성화간조직MDA함량승고,SOD활성강저(P＜0.05혹0.01)；여LT조비교,EP조혈청ALT、AST적활성화간조직MDA함량강저,SOD활성승고(P＜0.05혹0.01).결론 병동산을지예선급약가감경원위간이식술대서간손상.
Objective To investigate the effects of ethyl pyruvate (EP) pretreatment on the liver injury in rats undergoing orthotopic liver transplantation (OLT).Methods Forty male adult SD rats,weighing 220-250 g,served as liver transplant donors and recipients.The recipient rats were randomly divided into 3 groups ( n =8 each):sham operation group (group S),OLT group and EP group.Group S only underwent simple laparotomy.The model of OLT was established according to the modified Kamada's two-cuff technique in groups OLT and EP.EP 40 mg/kg was injected via the caudal vein at 1 h before skin incision in group EP.Venous blood samples were taken at 2 h of neohepatic phase to determine the serum activities of alanine amino-transferase (ALT) and aspartate amino-transferase (AST).Hepatic specimens were obtained to detect the content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD).Results Compared with group S,the levels of serum ALT and AST and MDA were significantly increased and the activity of SOD was significantly decreased in groups OLT and EP ( P ＜0.05 or 0.01).The levels of serum ALT and AST and MDA were significantly lower and the activity of SOD was significantly higher in group EP than in group OLT ( P ＜ 0.05 or 0.01 ).Conclusion Ethyl pyruvate pretreatment can attenuate the liver injury in rats undergoing OLT.