CAJ | 학술논문

目的研究焦炉工尿中1-羟基芘水平与外周血淋巴细胞胞质分裂阻滞微核(CBMN)率的关系.方法分别选取158名焦炉作业工人(接触组)和158名非职业接触人员(对照组),使用碱水解-高效液相色谱法测定尿中1-羟基芘浓度作为个体多环芳烃接触内剂量,应用CBMN法评价研究对象外周血淋巴细胞染色体损伤,使用调查表收集对象的职业接触史、年龄、性别、吸烟和饮酒状况等信息.结果调整了年龄、性别、吸烟和饮酒状况后,接触组外周血淋巴细胞CBMN率(3.32‰±2.90‰)明显高于对照组(0.57‰±0.88‰),差异有统计学意义(P＜0.01),呈炉顶工＞炉侧工＞炉底工＞对照组的分布,组间比较,差异无统计学意义(P＞0.05).且接触组CBMN率与尿中1-羟基芘水平呈正相关(r=0.28,P＜0.05).进一步按尿中1-羟基芘水平将研究对象分成0.11～0.70(104人)、0.71～4.09(107人),4.10～24.74 μmol/mol Cr组(105人)3个内剂量组,使用多元非参数协方差分析校正了不同内剂量组个体的年龄、性别、吸烟和饮酒状况后,0.71～4.09 μmol/mol Cr组的CBMN率为1.89‰±2.37‰,4.10～24.74μmol/mol Cr组的CBMN率为3.29‰±2.36‰,均明显高于0.11～0.70μmol/mol Cr组0.56‰±0.89‰,差异有统计学意义(P＜0.05).接触工龄和吸烟对CBMN率无影响.结论焦炉工外周血淋巴细胞CBMN率随尿中1-羟基芘浓度的增加而明显增加.
목적 연구초로공뇨중1-간기비수평여외주혈림파세포포질분렬조체미핵(CBMN)솔적관계.방법 분별선취158명초로작업공인(접촉조)화158명비직업접촉인원(대조조),사용감수해-고효액상색보법측정뇨중1-간기비농도작위개체다배방경접촉내제량,응용CBMN법평개연구대상외주혈림파세포염색체손상,사용조사표수집대상적직업접촉사、년령、성별、흡연화음주상황등신식.결과 조정료년령、성별、흡연화음주상황후,접촉조외주혈림파세포CBMN솔(3.32‰±2.90‰)명현고우대조조(0.57‰±0.88‰),차이유통계학의의(P＜0.01),정로정공＞로측공＞로저공＞대조조적분포,조간비교,차이무통계학의의(P＞0.05).차접촉조CBMN솔여뇨중1-간기비수평정정상관(r=0.28,P＜0.05).진일보안뇨중1-간기비수평장연구대상분성0.11～0.70(104인)、0.71～4.09(107인),4.10～24.74 μmol/mol Cr조(105인)3개내제량조,사용다원비삼수협방차분석교정료불동내제량조개체적년령、성별、흡연화음주상황후,0.71～4.09 μmol/mol Cr조적CBMN솔위1.89‰±2.37‰,4.10～24.74μmol/mol Cr조적CBMN솔위3.29‰±2.36‰,균명현고우0.11～0.70μmol/mol Cr조0.56‰±0.89‰,차이유통계학의의(P＜0.05).접촉공령화흡연대CBMN솔무영향.결론 초로공외주혈림파세포CBMN솔수뇨중1-간기비농도적증가이명현증가.
Objective To investigate the relationship between the urinary 1-hydroxypyrene level and cytokinesis-block micronucleus (CBMN)in peripheral blood lymphocyte in coke oven workers. Methods One hundred and fifty-eight workers from a coke plant and 158 referents without occupational PAHs exposure were recruited in this study. Urnary level of 1-hydroxypyrene was measured by alkaline hydrolysis combined with high performance liquid chromatography as an internal exposure dose, and the chromosomal damage of peripheral blood lymphocyte were evaluated with CBMN method. Personal information including occupational history, age, gender, smoking and alcohol drinking, was collected by questionnaire. Results The lymphocyte chromosomal damage level expressed as frequency of CBMN in coke oven workers was significantly higher than that of controls (3.32‰±2.90‰ vs 0.57‰±0.88‰, P＜0.01 ) after adjusting for gender, age, smoking and alcohol drinking, and correlation between urinary 1-hydroxypyrene concentrations and frequency of CBMN was found (Spearman partial correlation coefficient = 0.28, P＜0.05 ) in coke oven workers. Three hundreds and sixteen subjects were divided into three groups by their urine 1-hydroxypyrene level (expressed as 0.11 ～0.70,0.71～4.09 and 4.10～24.74 μmol/mol Cr). After adjusting for age, gender, smoking and alcohol drinking by multiple nonparametric analysis of covariance, the frequency of CBMN in the groups of 0.71 ～4.09 and 4.10～24.74 μmol/mol Cr were 1.89‰±2.37‰ and 3.29‰±2.36‰, significantly higher than that in the group of 0～0.70 μmol/mol Cr (0.56‰±0.89‰) (P＜0.05). Conclusions Under present PAHs exposure levels, CBMN test could detect PAHs-induced genotoxicity in coke oven workers.