CAJ | 학술논문

目的探讨接尘工人和尘肺患者痰细胞细胞间黏附因子-1(CD54,又称ICAM-1)、表皮生长因子受体(EGFR)、血小板衍生因子(PDGF)对尘肺早期诊断的意义.方法选择接尘工人62名为接尘组,非接尘工人51名为非接尘组,22例尘肺患者为尘肺组,健康检查者10例为健康对照组,用呼吸道诱痰技术收集研究对象的痰液,并利用流式细胞技术测定细胞表面标记物CD54、EGFR、PDGF的表达水平.结果接尘组与非接尘组痰细胞(中性粒细胞、巨噬细胞、淋巴细胞及嗜酸/碱粒细胞)表面标记物CD54、EGFR、PDGF的表达水平比较,差异无统计学意义(P＞0.05).与其他接尘工龄工人比较,接尘工龄10年及以上的工人痰细胞表面CD54、EGFR表达水平明显升高,PDGF表达水平明显降低,差异均有统计学意义(P＜0.05).与健康对照组比较,Ⅰ期、Ⅱ+Ⅲ期尘肺患者痰细胞CD54、EGFR表达水平明显升高,PDGF表达水平明显降低,差异均有统计学意义(P＜0.05).结论接尘工人和尘肺患者的痰细胞表面CD54、EGFR、PDGF表达水平有改变,可能对早期检测尘肺有重要意义.
목적 탐토접진공인화진폐환자담세포세포간점부인자-1(CD54,우칭ICAM-1)、표피생장인자수체(EGFR)、혈소판연생인자(PDGF)대진폐조기진단적의의.방법 선택접진공인62명위접진조,비접진공인51명위비접진조,22례진폐환자위진폐조,건강검사자10례위건강대조조,용호흡도유담기술수집연구대상적담액,병이용류식세포기술측정세포표면표기물CD54、EGFR、PDGF적표체수평.결과 접진조여비접진조담세포(중성립세포、거서세포、림파세포급기산/감립세포)표면표기물CD54、EGFR、PDGF적표체수평비교,차이무통계학의의(P＞0.05).여기타접진공령공인비교,접진공령10년급이상적공인담세포표면CD54、EGFR표체수평명현승고,PDGF표체수평명현강저,차이균유통계학의의(P＜0.05).여건강대조조비교,Ⅰ기、Ⅱ+Ⅲ기진폐환자담세포CD54、EGFR표체수평명현승고,PDGF표체수평명현강저,차이균유통계학의의(P＜0.05).결론 접진공인화진폐환자적담세포표면CD54、EGFR、PDGF표체수평유개변,가능대조기검측진폐유중요의의.
Objective To explore the significance of intercellular adhesion molecule -1 (CD54 or ICAM-1 ),epidermal growth factor receptor (EGFR) and platelet-derived factor (PDGF) in sputum cells of workers exposed to dust and patients with pneumoconiosis for the early diagnosis of pneumoconiosis.Methods The subjects included 62 workers exposed to dusts,51 workers not exposed to dusts,22 patients with pneumoconiosis and 10 healthy controls.The respiratory sputum technique was used to collect the sputum samples and the biomarkers (ICAM-1,EGFR and PDGF) of the sputum samples were detected with the sputum samples.Results When the exposure group was compared with non-exposure group,there were no significant differences of surface biomarkers (ICAM-1,EGFR and PDGF ) in sputum cells (neutrophil leucocytes,macrophages,lymphocytes and acidophilic/basophil leucocytes).As compared with other workers exposed to dusts,the surface CD54 and EGFR expression levels increased significantly and the surface PDGF expression level decreased significantly in workers exposed to dusts for 10 years (P＜0.05).As compared with controls,the CD54 and EGFR expression levels of sputum cells increased significantly and the PDGF expression level of sputum cells decreased significantly in patients with pneumoconiosis at the stages of Ⅰ and Ⅱ + Ⅲ (P＜0.05).Conclusion The expression levels of the surface CD54,EGFR and PDGF of sputum cells in workers exposed to dusts and patients with pneumoconiosis changed,which may be useful for early detecting pneumoconiosis.and patients is changed,which may be meaningful for early detection of pneumoconiosis.