CAJ | 학술논문

目的评价氯法齐明对不同耐药类型的MTB临床分离菌株的体外抑菌活性,为临床应用提供依据.方法应用微孔板观察法,测定氯法齐明对MTB标准株H37Rv及临床分离敏感、单耐药、多耐药、耐多药和广泛耐药菌株各15株的MIC,并将氯法齐明对不同耐药类型的MTB菌株体外活性与异烟肼、利福平、阿米卡星、卷曲霉素和氧氟沙星进行比较.结果氯法齐明对敏感菌株的MIC(0.06～4.00mg/L)高于异烟肼(0.06～0.25 mg/L)和利福平(0.06～0.25 mg/L),与氧氟沙星(0.06～2.00mg/L)、阿米卡星(0.06～4.00mg/L)和卷曲霉素(0.50～4.00mg/L)相似;氯法齐明对单耐药菌株的MIC(0.03～4.00mg/L)与异烟肼(0.06～0.25 mg/L)和利福平(0.06～0.25 mg/L)相似,低于氧氟沙星(0.25～8.00mg/L)、阿米卡星(0.06～4.00mg/L)和卷曲霉素(0.50～8.00mg/L);氯法齐明对耐多药菌株的MIC(0.06～8.00mg/L)与阿米卡星(0.25～8.00mg/L)相似,优于氧氟沙星(0.125～8.00mg/L)和卷曲霉素(0.50～8.00mg/L);氯法齐明对广泛耐药菌株的MIC(0.03～8.00mg/L)明显优于其他药物.结论氯法齐明对MTB菌株,尤其是耐多药和广泛耐药MTB菌株,均有较好的体外抑菌活性.
목적 평개록법제명대불동내약류형적MTB림상분리균주적체외억균활성,위림상응용제공의거.방법 응용미공판관찰법,측정록법제명대MTB표준주H37Rv급림상분리민감、단내약、다내약、내다약화엄범내약균주각15주적MIC,병장록법제명대불동내약류형적MTB균주체외활성여이연정、리복평、아미잡성、권곡매소화양불사성진행비교.결과 록법제명대민감균주적MIC(0.06～4.00mg/L)고우이연정(0.06～0.25 mg/L)화리복평(0.06～0.25 mg/L),여양불사성(0.06～2.00mg/L)、아미잡성(0.06～4.00mg/L)화권곡매소(0.50～4.00mg/L)상사;록법제명대단내약균주적MIC(0.03～4.00mg/L)여이연정(0.06～0.25 mg/L)화리복평(0.06～0.25 mg/L)상사,저우양불사성(0.25～8.00mg/L)、아미잡성(0.06～4.00mg/L)화권곡매소(0.50～8.00mg/L);록법제명대내다약균주적MIC(0.06～8.00mg/L)여아미잡성(0.25～8.00mg/L)상사,우우양불사성(0.125～8.00mg/L)화권곡매소(0.50～8.00mg/L);록법제명대엄범내약균주적MIC(0.03～8.00mg/L)명현우우기타약물.결론 록법제명대MTB균주,우기시내다약화엄범내약MTB균주,균유교호적체외억균활성.
Objective To study the in vitro antituberculous activities of clofazimine (CLF) to different drug-resistant types of Mycobacterium tuberculosis.Methods The minimal inhibitory concentration (MIC) of CLF and isoniazid (INH), rifampicin (RFP), ofloxacin (OFLX), amikacin (AK), and capreomycin (CPM) against sensitive, single-drug resistant (SDR), poly-drug resistant (PDR), multi-drug resistant (MDR), and extensive-drug resistant (XDR) Mycobacterium tuberculosis strains isolated clinically were determined by microplate assays.Results The MICs of CLF for sensitive, SDR, PDR, MDR and XDR strains of clinically isolated Mycobacterium tuberculosis were 0.06 -4.00 mg/L, 0.03 -4.00 mg/L, 0.06 -8.00 mg/L, 0.06 - 8.00 mg/L, 0.03 - 8.00 mg/L.For the sensitive group, the MIC of CLF ( 0.06 -4.00 mg/L) was higher than that of INH (0.06 -0.25 mg/L) and RFP (0.06 -0.25 mg/L), while there was no significant difference among OFLX (0.06 -2.00 mg/L), AK (0.06 -4.00 mg/L), and CPM (0.50 -4.00 mg/L).For the single-drug resistant group, there was no significant difference among CLF (0.03-4.00 mg/L), INH (0.06-0.25 mg/L), and RFP (0.06-0.25 mg/L), but the MIC of CLF was lower than that of OFLX ( 0.25 - 8.00 mg/L), AK ( 0.06 - 4.00 mg/L ), and CPM ( 0.50 - 8.00 mg/L).For the MDR group, there was no significant difference between CLF (0.06 -8.00 mg/L) and AK (0.25 - 8.00 mg/L), but the MIC of CLF was lower than that of OFLX (0.125 - 8.00 mg/L) and CPM (0.50 -8.O0 mg/L).For the XDR group, the MIC of CLF (0.03 -8.00 mg/L) was lower than that of others.Conclusion CLF showed good in vitro activity against Mycobacterium tuberculosis, especially MDR and XDR strains.