中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2012年
6期
324-328
,共5页
血管瘤%细胞系%平阳霉素
血管瘤%細胞繫%平暘黴素
혈관류%세포계%평양매소
Hemangioma%Cell line%Bleomycin A5
目的 探讨平阳霉素( bleomycin A5)对婴幼儿血管瘤细胞系XTPS-1作用.方法 婴幼儿血管瘤源性血管内皮细胞系XTPS-1经不同质量浓度(1000、100、10、1、0 mg/L)的平阳霉素处理后,利用倒置相差显微镜和电子显微镜观察其形态学特征的变化,采用甲基噻唑基四唑( methylthiazolyl terazolium,MTT)法观察XTPS-1存活率,流式细胞仪检测其周期和凋亡率,5组均数之间比较用单因素方差分析,两组间比较用t检验.结果 低质量浓度组(10 mg/L和1 mg/L组)细胞毒性作用非常弱,24h存活率为(92.96±3.66)％和(99.86±0.12)％；尤其是1 mg/L组基本没有细胞毒性作用,但是具有诱导细胞凋亡的作用；高质量浓度组(1000 mg/L和100 mg/L组)既表现出较强的细胞毒性作用,而且细胞毒性作用呈浓度依赖性,24h存活率为(34.08±3.11)％和(43.28±2.88)％；又具有较强的诱导细胞凋亡作用.高质量浓度组与低质量浓度组相比,差异有统计学意义(P＜0.01).结论 平阳霉素对XTPS-1能诱发细胞凋亡,抑制细胞的增殖,较高质量浓度的平阳霉素还具有直接细胞毒性作用,细胞毒性作用呈浓度依赖性.
目的 探討平暘黴素( bleomycin A5)對嬰幼兒血管瘤細胞繫XTPS-1作用.方法 嬰幼兒血管瘤源性血管內皮細胞繫XTPS-1經不同質量濃度(1000、100、10、1、0 mg/L)的平暘黴素處理後,利用倒置相差顯微鏡和電子顯微鏡觀察其形態學特徵的變化,採用甲基噻唑基四唑( methylthiazolyl terazolium,MTT)法觀察XTPS-1存活率,流式細胞儀檢測其週期和凋亡率,5組均數之間比較用單因素方差分析,兩組間比較用t檢驗.結果 低質量濃度組(10 mg/L和1 mg/L組)細胞毒性作用非常弱,24h存活率為(92.96±3.66)％和(99.86±0.12)％；尤其是1 mg/L組基本沒有細胞毒性作用,但是具有誘導細胞凋亡的作用；高質量濃度組(1000 mg/L和100 mg/L組)既錶現齣較彊的細胞毒性作用,而且細胞毒性作用呈濃度依賴性,24h存活率為(34.08±3.11)％和(43.28±2.88)％；又具有較彊的誘導細胞凋亡作用.高質量濃度組與低質量濃度組相比,差異有統計學意義(P＜0.01).結論 平暘黴素對XTPS-1能誘髮細胞凋亡,抑製細胞的增殖,較高質量濃度的平暘黴素還具有直接細胞毒性作用,細胞毒性作用呈濃度依賴性.
목적 탐토평양매소( bleomycin A5)대영유인혈관류세포계XTPS-1작용.방법 영유인혈관류원성혈관내피세포계XTPS-1경불동질량농도(1000、100、10、1、0 mg/L)적평양매소처리후,이용도치상차현미경화전자현미경관찰기형태학특정적변화,채용갑기새서기사서( methylthiazolyl terazolium,MTT)법관찰XTPS-1존활솔,류식세포의검측기주기화조망솔,5조균수지간비교용단인소방차분석,량조간비교용t검험.결과 저질량농도조(10 mg/L화1 mg/L조)세포독성작용비상약,24h존활솔위(92.96±3.66)％화(99.86±0.12)％；우기시1 mg/L조기본몰유세포독성작용,단시구유유도세포조망적작용；고질량농도조(1000 mg/L화100 mg/L조)기표현출교강적세포독성작용,이차세포독성작용정농도의뢰성,24h존활솔위(34.08±3.11)％화(43.28±2.88)％；우구유교강적유도세포조망작용.고질량농도조여저질량농도조상비,차이유통계학의의(P＜0.01).결론 평양매소대XTPS-1능유발세포조망,억제세포적증식,교고질량농도적평양매소환구유직접세포독성작용,세포독성작용정농도의뢰성.
Objective To investigate the effect of bleomycin A5 on the hemangioma-derived endothelial cell line XTPS-1.Methods Hemangioma-derived endothelial cell line XTPS-1 was cultured with different concentration of bleomycin A5 ( 1000,100,10,1,0 mg/L),and then the survival rate was measured by methyl thiazolyl terazolium (MTT),the variation of cell morphology was observed using inverted phase contrast microscope and electron microscope,the variation of cell cycle and apoptosis rate were measured using flow cytometry.Results After 24 hours culture the cell survival rate was ( 92.96±3.66 )％ and (99.86± 0.12)％ in lower saturation group(10 and 1 mg/L),but (34.08 ±3.11)％ and (43.28 ±2.88 )％ in higher saturation group( 1000 and 100 mg/L).The difference between them was more significant (P ＜ 0.01 ).Lower saturation of bleomycin A5 (10 and 1 mg/L)could induce apoptosis but had almost no cytotoxic effect.Higher saturation of bleomycin A5 ( 1000 and 100 mg/L) not only induced apoptosis,but also had strong cytotoxic effect,which was concentration dependent.Conclusions bleomycin A5 could induce apoptosis,inhibit cell proliferation and has direct cytotoxic effect.
