CAJ | 학술논문

对42例肺癌患者和25例肺部良性病变患者行纤维支气管镜检查,并利用甲基化特异性PCR方法检测外周血血浆、痰液、支气管肺泡灌洗液中p16基因甲基化状态.42例确诊肺癌患者p16基因异常,甲基化阳性数在血浆、痰液和支气管肺泡灌洗液中分别为22例(52.4%)、20例(47.6%)和25例(59.5%),各标本的阳性率差异无统计学意义(P＞0.05);25例良性病变者中除1例在血液中检测到异常甲基化外,均未检测到p16基因的异常甲基化.经纤维支气管镜确诊25例,敏感度、特异性和准确性分别为59.5%、100.0%和74.6%;纤维支气管镜联合p16基因甲基化检测后敏感度、特异性和准确性分别为92.9%、%.2%和94.0%.
대42례폐암환자화25례폐부량성병변환자행섬유지기관경검사,병이용갑기화특이성PCR방법검측외주혈혈장、담액、지기관폐포관세액중p16기인갑기화상태.42례학진폐암환자p16기인이상,갑기화양성수재혈장、담액화지기관폐포관세액중분별위22례(52.4%)、20례(47.6%)화25례(59.5%),각표본적양성솔차이무통계학의의(P＞0.05);25례량성병변자중제1례재혈액중검측도이상갑기화외,균미검측도p16기인적이상갑기화.경섬유지기관경학진25례,민감도、특이성화준학성분별위59.5%、100.0%화74.6%;섬유지기관경연합p16기인갑기화검측후민감도、특이성화준학성분별위92.9%、%.2%화94.0%.
Fibro-optic bronchoscopy (FB) examinations were undertaken in 42 cases with lung cancer and 25 cases with benign lung disease; methylation-specific PCR was performed in plasma, sputum and bronchoalveolar lavage fluid (BALF) specimen for detection of p16 gene promoter methylation in all patients.Of the 42 cases of lung cancer, the positive rates of p16 gene promoter methylation were 59.5% in BALF, 52.4% in plasma and 47.6% in sputum, respectively; while p16 gene promoter methylation was detected only in one plasma sample from 25 cases with benign lung disease ( P ＜ 0.05 ).The sensitivity,specificity and overall accuracy of FB were 59.5%, 100.0% and 74.6%, respectively.The sensitivity,specificity and overall accuracy of FB combined with aberrant p16 gene methylation in diagnosis of lung cancer were 92.9%, 96.2% and 94.0%, respectively.The FB examinations combined with detection of aberrant p16 gene methylation can further improve the accuracy to diagnosis of lung cancer.