CAJ | 학술논문

目的探讨Wistar大鼠肾缺血再灌注损伤模型对大鼠肾近曲小管上皮表达CD133和CD34抗原细胞的影响及抗原分布,为肾成体干细胞研究提供参考.方法通过手术夹闭大鼠双侧肾动脉约50 min后再次开放血流,形成肾缺血再灌注损伤模型.术后第3、5、7大取肾脏进行免疫组织化学检测,观察在肾近曲小管上皮细胞中肾损伤因子(KIM)1、CD34、CD133、5-溴2-脱氧尿嘧啶核苷(Brdu)抗原表达及其阳性表达的该细胞亚群的分布及时间变迁.对照组除不夹闭肾动脉外,其余操作同实验组.结果与对照组相比,实验组大鼠KIM-1和CD133阳性细胞主要集中在肾皮质,CD34、Brdu阳性细胞主要集中在皮髓质交界处和髓质.术后第3、5、7天,KIM-1及CD133抗原阳性表达强度为先增加再减少(KIM-1为40.3%±3.2%,57.5%±3.8%,24.3%±1.4%;CD133为23.4%±2.2%,34.3%±3.1%,16.6%±1.8%);CD34抗原为术后逐渐减少(56.0%±4.8%,44.2%±2.2%,28.8%±1.0%);Brdu抗原为术后逐渐增加(10.0%±1.1%,36.0%±4.2%,48.8%±5.0%).结论缺血再灌注损伤之后,肾皮质中表达KIM-1和CD133抗原阳性的细胞明显增多,激发肾髓质中表达CD34抗原和Brdu阳性的细胞明显增多,继发增殖再生.肾髓质区有类似于上皮干/祖细胞抗原CD34和增殖抗原Brdu的细胞存在,提示很可能增殖和分化过程由髓质向皮质迁移,为探索肾成体干细胞研究及肾单位的组织重建可行性研究提供了依据.
목적 탐토Wistar대서신결혈재관주손상모형대대서신근곡소관상피표체CD133화CD34항원세포적영향급항원분포,위신성체간세포연구제공삼고.방법 통과수술협폐대서쌍측신동맥약50 min후재차개방혈류,형성신결혈재관주손상모형.술후제3、5、7대취신장진행면역조직화학검측,관찰재신근곡소관상피세포중신손상인자(KIM)1、CD34、CD133、5-추2-탈양뇨밀정핵감(Brdu)항원표체급기양성표체적해세포아군적분포급시간변천.대조조제불협폐신동맥외,기여조작동실험조.결과 여대조조상비,실험조대서KIM-1화CD133양성세포주요집중재신피질,CD34、Brdu양성세포주요집중재피수질교계처화수질.술후제3、5、7천,KIM-1급CD133항원양성표체강도위선증가재감소(KIM-1위40.3%±3.2%,57.5%±3.8%,24.3%±1.4%;CD133위23.4%±2.2%,34.3%±3.1%,16.6%±1.8%);CD34항원위술후축점감소(56.0%±4.8%,44.2%±2.2%,28.8%±1.0%);Brdu항원위술후축점증가(10.0%±1.1%,36.0%±4.2%,48.8%±5.0%).결론 결혈재관주손상지후,신피질중표체KIM-1화CD133항원양성적세포명현증다,격발신수질중표체CD34항원화Brdu양성적세포명현증다,계발증식재생.신수질구유유사우상피간/조세포항원CD34화증식항원Brdu적세포존재,제시흔가능증식화분화과정유수질향피질천이,위탐색신성체간세포연구급신단위적조직중건가행성연구제공료의거.
Objective To study the influence and distribution in proximal tubule epithelial cells with the expressions of CD133 and CD34 in a rat model so as to provide a study basis for renal adult stem cell. Methods The kidney ischemia/reperfusion model was established by blocking the bilateral renal arteries for about 50 min and recovering the renal perfusion of blood. Then the rat kidneys were extracted at Days 3, 5 and 7 post-modeling. After a series of special treatment, immunohistochemical staining was used to show the distribution and expression intensity of KIM-1, Brdu antigen, CD34 and CD133 antigens in cells with the elapsing of time. Results As compared with control group, the KIM-1 and CD133 antigens were present in cotex renis while the CD34 and Brdu antigens were distributed in parts of medulla renis and juncture of cotex-medulla renis. The expression density value of KIM-1 and CD133 antigens rose for the first 3 days then declined afterward ( 40. 3% ± 3.2%, 57. 5% ± 3. 8%, 24. 3% ± 1.4% ) . Otherwise the expression density value of CD 34 antigen declined(56. 0% ±4. 8% ,44. 2% ±2. 2% ,28. 8% ± 1.0% )and Brdu antigen showed an upward trend at post-operation( 10. 0% ± 1.1% ,36. 0% ±4. 2% ,48.8% ±5. 0% ).Conclusion After ischemia/reperfusion injury in kidney, the expression rates of CD133 and KIM-1 antigen rise obviously in cotex renis. And the D34 and Brdu antigens show a similar trend in medulla renis. The result indicates that the phenomenon of proliferation and differentiation may appears in kidney proximal tubule and migrate from the region of medulla renis to cotex renis. The participating cells not only possess the strong proliferation and repairing ability of stem/progenitor cells, but also can expresses the CD34 and CD133 antigens. Thus it is may provide a study basis for the tissue reconstruction of nephron and research in the field of kidney adult stem cell.