CAJ | 학술논문

目的探讨 CD34和CD31在不同亚型肾细胞癌(RCC)中的表达,及其标记的微血管密度(MVD)与RCC临床病理因素之间的关系.方法采用免疫组化SP法,检测CD34和CD31在149例RCC组织(肾透明细胞癌76例,肾乳头状癌42例,肾嫌色细胞癌31例)中的表达情况.结果肾透明细胞癌、肾嫌色细胞癌和肾乳头状癌组织中CD34 标记的MVD分别为128.04±46.44、48.55±14.09和38.12±10.98,CD31标记的MVD分别为98.35±55.05、30.70±17.72和21.60±9.38,肾透明细胞癌组织中CD34标记的MVD和CD31标记的MVD均明显高于肾非透明细胞癌组织(均P<0.01).在肾嫌色细胞癌和肾乳头状癌组织中,CD34标记的MVD均明显高于CD31 标记的MVD(均P<0.01).CD34和CD31标记的MVD均与肾透明细胞癌的组织学分级呈负相关(r_(CD34)=-0.618,P<0.01;r_(CD31)=-0.442,P<0.01),也与临床分期呈负相关(r_(CD34)=-0.283,P<0.05;r_(CD31)=-0.256,P<0.05),其中CD34的相关性较强;但与肾非透明细胞癌的组织学分级和临床分期无明显的相关性(均P>0.05).结论 CD34 和 CD31均能清晰地、选择性地显示RCC的MVD,但CD34比CD31更敏感.肾透明细胞癌的MVD显著高于肾非透明细胞癌.肾透明细胞癌的MVD与组织学分级和临床分期呈负相关,而肾非透明细胞癌的MVD与组织学分级和临床分期并无相关性.
목적 탐토 CD34화CD31재불동아형신세포암(RCC)중적표체,급기표기적미혈관밀도(MVD)여RCC림상병리인소지간적관계.방법 채용면역조화SP법,검측CD34화CD31재149례RCC조직(신투명세포암76례,신유두상암42례,신혐색세포암31례)중적표체정황.결과 신투명세포암、신혐색세포암화신유두상암조직중CD34 표기적MVD분별위128.04±46.44、48.55±14.09화38.12±10.98,CD31표기적MVD분별위98.35±55.05、30.70±17.72화21.60±9.38,신투명세포암조직중CD34표기적MVD화CD31표기적MVD균명현고우신비투명세포암조직(균P<0.01).재신혐색세포암화신유두상암조직중,CD34표기적MVD균명현고우CD31 표기적MVD(균P<0.01).CD34화CD31표기적MVD균여신투명세포암적조직학분급정부상관(r_(CD34)=-0.618,P<0.01;r_(CD31)=-0.442,P<0.01),야여림상분기정부상관(r_(CD34)=-0.283,P<0.05;r_(CD31)=-0.256,P<0.05),기중CD34적상관성교강;단여신비투명세포암적조직학분급화림상분기무명현적상관성(균P>0.05).결론 CD34 화 CD31균능청석지、선택성지현시RCC적MVD,단CD34비CD31경민감.신투명세포암적MVD현저고우신비투명세포암.신투명세포암적MVD여조직학분급화림상분기정부상관,이신비투명세포암적MVD여조직학분급화림상분기병무상관성.
Objective The aim of this study was to evaluate the expressions of CD34, CD31 and microvessel density (MVD) in different subtypes of renal cell carcinoma (RCC) as well as the relationship between MVD and clinicopathological factors. Methods Expressions of CD31 and CD34 were detected in 149 patients with RCC using SP immunohistochemical staining. The MVD was studied by Weidner's method. Results The expressions of CD31 and CD34 in the clear cell renal cell carcinoma (CCRCC) (98.35± 55.05, 128.04±46.44) were significantly higher than those in chromophobe renal cell carcinoma (ChRCC) (30.70±17.72, 48.55±14.09) and papillary renal cell carcinoma ( PRCC ) (21.60±9.38, 38.12±10.98 ) (P<0.01). The MVD value marked by CD31 (30.70±17.72, 21.60±9.38 ) was much lower than that marked by CD34 (48.55±14.09, 38.12±10.98) between ChRCC and PRCC (P < 0.01 ). Smaller and immatured microvessels and even single endothelial cells could be clearly seen. The MVD values marked by CD31 and CD34 were negatively correlated with the pathological grades (r_(CD34) =-0.618, P<0.01 ; r_(CD31) =-0. 442,P<0.01) and clinical stages (r_(CD34)=-0.283 ,P<0.05;r_(CD31)=-0. 256 ,P < 0.05) in CCRCC. But no association was found in non-CCRCC ( P>0.05 ). Conclusion MVD is significantly correlated with different types of endothelial labeling. The microvascular endothelial cells could be shown clearly by its related antigen labeling such as CD34 and CD31.CD34 is more sensitive than CD31. The MVD of CCRCC is significantly higher than that in non-CCRCC. The expressions of CD31 and CD34 are not correlated with tumor grade and stage in ChRCC and PRCC, while there is a negative correlation in CCRCC.