CAJ | 학술논문

Summary: The effects of inhibitors of TNFα converting enzyme (TACE) on TNFa secretion werestudied to develop an approach to interfere inflammation processes. The HL-60 cell lines were stimu-lated in vitro with LPS intravenously for different time to establish the cellular model of inflammationand simultaneously induce in vivo inflammation animal model by LPS The cytotoxic effects of solubleTNFα were checked using MTT colorimetric method to determine the rate of cell proliferation. Thelevel of expression of TACE was detected by using RT-PCR, FCM and immuno-histochemical tech-nique respectively. It was found Chinese medicine Reduqing (RDQ) could inhibit the transcription ofTNFα mRNA induced by LPS stimulation (P＜0. 01, compared with the control). The anti-oligodeoxyribonucleotide (anti-ODN) of TNFα mRNA could inhibit 78.9 % of TNFα secretion. Themimic peptides of TACE substrates with hydroxamine group showed potency in vivo and in vitro a-gainst converting of pro-TNFα. It was concluded that all the three types of TACE inhibitors can reg-ulate the expression of TACE at different levels and inhibit sTNFα secretion, indicating TACE is anovel target for inflammation therapy.