CAJ | 학술논문

利用刺囊毛霉(Mucor spinosus)3.3450的培养液对天麻素进行生物转化,得到其酶水解产物甙元对羟基苯甲醇,转化率接近100%.通过无细胞抽提液对底物转化的研究,证明催化该反应的酶为组成酶、胞外酶.
이용자낭모매(Mucor spinosus)3.3450적배양액대천마소진행생물전화,득도기매수해산물대원대간기분갑순,전화솔접근100%.통과무세포추제액대저물전화적연구,증명최화해반응적매위조성매、포외매.
Microbial transformation of gastrodin by Mucor spinosus strain 3.3450, resulted in a product with a transformation rate close to 100 per cent. This product was identified as p-hydroxy benzyl alcohol on the basis of its 1H, 13C NMR and EI-MS spectral data. It could be inferred that the enzyme responsible for the biotransformation reaction was a kind of extracellular and constitutive enzyme since the transformation reaction of the substrate could be carried out in cell free extracts of the fermentation broth of the Mucor spinosus.