CAJ | 학술논문

背景与目的:了解红曲黄色素对机体是否具有毒性作用. 材料与方法:选用清洁级SD大鼠,试验设3个红曲黄色素剂量组(0.23'、0.47、0.93 g/kg)及空白对照组(基础饲料).试验期间观察动物形态,每周称重,记录饲料消耗量.第45 d时,采尾血测血常规、血生化;喂养90 d后,摘眼球采血作血常规及生化指标测定,然后取出肝、肾、脾、睾丸称重,并对肝、肾、脾、胃肠、睾丸、卵巢作病理学检查.结果:实验期间3个红曲黄色素组的大鼠体重增加、饲料利用率与对照组相比差异均无统计学意义(P>0.05).喂养45 d和90 d后,3个红曲黄色素组大鼠的血红蛋白含量、红细胞、白细胞计数及白细胞分类均在正常值范围内.其血清谷丙转氨酶、谷草转氨酶、尿素、肌酐、总胆固醇、甘油三酯、血糖、总蛋白、白蛋白测定值均在正常值范围内.且3个红曲黄色素组大鼠的肾体比、脾体比、肝体比、睾体比与对照组比较差异均无统计学意义(P>0.05).组织病理学检查结果显示各剂量组大鼠的肝、肾、胃、脾切片染色后在光镜下检查未见特异性病理改变.结论:在本实验条件下,红曲黄色素喂养90 d对大鼠未产生毒性作用,提示可作为天然色素在食品加工生产中在安全的剂量范围内可适当使用.
배경여목적:료해홍곡황색소대궤체시부구유독성작용. 재료여방법:선용청길급SD대서,시험설3개홍곡황색소제량조(0.23'、0.47、0.93 g/kg)급공백대조조(기출사료).시험기간관찰동물형태,매주칭중,기록사료소모량.제45 d시,채미혈측혈상규、혈생화;위양90 d후,적안구채혈작혈상규급생화지표측정,연후취출간、신、비、고환칭중,병대간、신、비、위장、고환、란소작병이학검사.결과:실험기간3개홍곡황색소조적대서체중증가、사료이용솔여대조조상비차이균무통계학의의(P>0.05).위양45 d화90 d후,3개홍곡황색소조대서적혈홍단백함량、홍세포、백세포계수급백세포분류균재정상치범위내.기혈청곡병전안매、곡초전안매、뇨소、기항、총담고순、감유삼지、혈당、총단백、백단백측정치균재정상치범위내.차3개홍곡황색소조대서적신체비、비체비、간체비、고체비여대조조비교차이균무통계학의의(P>0.05).조직병이학검사결과현시각제량조대서적간、신、위、비절편염색후재광경하검사미견특이성병리개변.결론:재본실험조건하,홍곡황색소위양90 d대대서미산생독성작용,제시가작위천연색소재식품가공생산중재안전적제량범위내가괄당사용.
BACKGROUND AND AIM: To investigate the toxicity in SD rats fed continuously with monascus yellow pigment. MATERIALS AND METHODS: SD rats were divided into four groups (0.23、0.47、0.93 g/kg and a blank control) and fed continually for 90 days. During the experimental period, rats were weighed once every week, general conditions were assessed, and feed consumptions were recorded. On the 45th day, blood was collected from tails to perform complete blood count and biochemical tests. After 90 days, eyeballs were extracted and blood was used for blood count and biochemical tests; livers, kidneys, spleens and testicles were weighed; and pathological examinations of livers, kidneys, spleens, stomach intestines, testicles and ovaries were done. RESULTS: The body weight increase and feed efficiency of all dose groups were not significantly different from those of control group(P> 0.05) .Complete blood count: the hemoglobin content, erythrocyte count, leukocyte count and differential in all dose groups were normal.Blood chemical test:serum ALT, AST, urea, creatinine, total cholesterol, triglyceride, glucose, total protein and albumin in all dose groups were normal. Organ/body weight ratios: the ratios of kidney/body, spleen/body, liver/body, testicle/body of all dose groups were not significantly different from those of control group(P> 0.05) .Pathological examination: no special pathological changes were found in the livers, kidneys, stomachs and spleens of rats in all dose groups. CONCLUSION: No toxic effects were observed in rats continuously fed with monascus yellow pigment for 90 days, suggesting it could be used as a natural pigment in food production.