癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
2009年
4期
316-319
,共4页
陈冠敏%林蔚%林春芳%黄宗锈%郑丽红%黄佳宁%陈秀锦
陳冠敏%林蔚%林春芳%黃宗鏽%鄭麗紅%黃佳寧%陳秀錦
진관민%림위%림춘방%황종수%정려홍%황가저%진수금
红曲黄色素%血常规%血生化%体重%脏体比%毒性
紅麯黃色素%血常規%血生化%體重%髒體比%毒性
홍곡황색소%혈상규%혈생화%체중%장체비%독성
monascus yellow pigment%complete blood count%blood biochemical test%body weight%organ /body weight ratio%toxicity
背景与目的:了解红曲黄色素对机体是否具有毒性作用. 材料与方法:选用清洁级SD大鼠,试验设3个红曲黄色素剂量组(0.23'、0.47、0.93 g/kg)及空白对照组(基础饲料).试验期间观察动物形态,每周称重,记录饲料消耗量.第45 d时,采尾血测血常规、血生化;喂养90 d后,摘眼球采血作血常规及生化指标测定,然后取出肝、肾、脾、睾丸称重,并对肝、肾、脾、胃肠、睾丸、卵巢作病理学检查.结果:实验期间3个红曲黄色素组的大鼠体重增加、饲料利用率与对照组相比差异均无统计学意义(P>0.05).喂养45 d和90 d后,3个红曲黄色素组大鼠的血红蛋白含量、红细胞、白细胞计数及白细胞分类均在正常值范围内.其血清谷丙转氨酶、谷草转氨酶、尿素、肌酐、总胆固醇、甘油三酯、血糖、总蛋白、白蛋白测定值均在正常值范围内.且3个红曲黄色素组大鼠的肾体比、脾体比、肝体比、睾体比与对照组比较差异均无统计学意义(P>0.05).组织病理学检查结果显示各剂量组大鼠的肝、肾、胃、脾切片染色后在光镜下检查未见特异性病理改变.结论:在本实验条件下,红曲黄色素喂养90 d对大鼠未产生毒性作用,提示可作为天然色素在食品加工生产中在安全的剂量范围内可适当使用.
揹景與目的:瞭解紅麯黃色素對機體是否具有毒性作用. 材料與方法:選用清潔級SD大鼠,試驗設3箇紅麯黃色素劑量組(0.23'、0.47、0.93 g/kg)及空白對照組(基礎飼料).試驗期間觀察動物形態,每週稱重,記錄飼料消耗量.第45 d時,採尾血測血常規、血生化;餵養90 d後,摘眼毬採血作血常規及生化指標測定,然後取齣肝、腎、脾、睪汍稱重,併對肝、腎、脾、胃腸、睪汍、卵巢作病理學檢查.結果:實驗期間3箇紅麯黃色素組的大鼠體重增加、飼料利用率與對照組相比差異均無統計學意義(P>0.05).餵養45 d和90 d後,3箇紅麯黃色素組大鼠的血紅蛋白含量、紅細胞、白細胞計數及白細胞分類均在正常值範圍內.其血清穀丙轉氨酶、穀草轉氨酶、尿素、肌酐、總膽固醇、甘油三酯、血糖、總蛋白、白蛋白測定值均在正常值範圍內.且3箇紅麯黃色素組大鼠的腎體比、脾體比、肝體比、睪體比與對照組比較差異均無統計學意義(P>0.05).組織病理學檢查結果顯示各劑量組大鼠的肝、腎、胃、脾切片染色後在光鏡下檢查未見特異性病理改變.結論:在本實驗條件下,紅麯黃色素餵養90 d對大鼠未產生毒性作用,提示可作為天然色素在食品加工生產中在安全的劑量範圍內可適噹使用.
배경여목적:료해홍곡황색소대궤체시부구유독성작용. 재료여방법:선용청길급SD대서,시험설3개홍곡황색소제량조(0.23'、0.47、0.93 g/kg)급공백대조조(기출사료).시험기간관찰동물형태,매주칭중,기록사료소모량.제45 d시,채미혈측혈상규、혈생화;위양90 d후,적안구채혈작혈상규급생화지표측정,연후취출간、신、비、고환칭중,병대간、신、비、위장、고환、란소작병이학검사.결과:실험기간3개홍곡황색소조적대서체중증가、사료이용솔여대조조상비차이균무통계학의의(P>0.05).위양45 d화90 d후,3개홍곡황색소조대서적혈홍단백함량、홍세포、백세포계수급백세포분류균재정상치범위내.기혈청곡병전안매、곡초전안매、뇨소、기항、총담고순、감유삼지、혈당、총단백、백단백측정치균재정상치범위내.차3개홍곡황색소조대서적신체비、비체비、간체비、고체비여대조조비교차이균무통계학의의(P>0.05).조직병이학검사결과현시각제량조대서적간、신、위、비절편염색후재광경하검사미견특이성병리개변.결론:재본실험조건하,홍곡황색소위양90 d대대서미산생독성작용,제시가작위천연색소재식품가공생산중재안전적제량범위내가괄당사용.
BACKGROUND AND AIM: To investigate the toxicity in SD rats fed continuously with monascus yellow pigment. MATERIALS AND METHODS: SD rats were divided into four groups (0.23、0.47、0.93 g/kg and a blank control) and fed continually for 90 days. During the experimental period, rats were weighed once every week, general conditions were assessed, and feed consumptions were recorded. On the 45th day, blood was collected from tails to perform complete blood count and biochemical tests. After 90 days, eyeballs were extracted and blood was used for blood count and biochemical tests; livers, kidneys, spleens and testicles were weighed; and pathological examinations of livers, kidneys, spleens, stomach intestines, testicles and ovaries were done. RESULTS: The body weight increase and feed efficiency of all dose groups were not significantly different from those of control group(P> 0.05) .Complete blood count: the hemoglobin content, erythrocyte count, leukocyte count and differential in all dose groups were normal.Blood chemical test:serum ALT, AST, urea, creatinine, total cholesterol, triglyceride, glucose, total protein and albumin in all dose groups were normal. Organ/body weight ratios: the ratios of kidney/body, spleen/body, liver/body, testicle/body of all dose groups were not significantly different from those of control group(P> 0.05) .Pathological examination: no special pathological changes were found in the livers, kidneys, stomachs and spleens of rats in all dose groups. CONCLUSION: No toxic effects were observed in rats continuously fed with monascus yellow pigment for 90 days, suggesting it could be used as a natural pigment in food production.