CAJ | 학술논문

目的探讨血小板活化因子乙酰水解酶(PAF-AH)基因Val379Ala位点多态性与脓毒症易感性及预后的关系.方法采用PCR-RFLP技术,分析82例不同程度脓毒症患者及90名健康对照者PAF-AH基因Val379Ala位点的多态性,对含有等位基因多态性的片段进行DNA测序分析.结果全部样本经PCR-RFLP分析后,脓毒症组82例中,仅1例纯合子Val/Val型,24例杂合子Val/Ala型,57例纯合子Ala/Ala型;对照组90例中,2例Val/Val型,29例Val/Ala型,59例Ala/Ala型.Val379Ala基因型和等位基因频率:脓毒症组与对照组比较差异无统计学意义(P>0.05),存活组、死亡组与对照组比较差异无统计学意义(P>0.05),轻型脓毒症组、严重脓毒症组与对照组比较差异无统计学意义(P>0.05).结论 PAF-AH基因Val379Ala位点多态性与脓毒症易感性、预后及病情严重程度可能不相关.
목적 탐토혈소판활화인자을선수해매(PAF-AH)기인Val379Ala위점다태성여농독증역감성급예후적관계.방법 채용PCR-RFLP기술,분석82례불동정도농독증환자급90명건강대조자PAF-AH기인Val379Ala위점적다태성,대함유등위기인다태성적편단진행DNA측서분석.결과 전부양본경PCR-RFLP분석후,농독증조82례중,부1례순합자Val/Val형,24례잡합자Val/Ala형,57례순합자Ala/Ala형;대조조90례중,2례Val/Val형,29례Val/Ala형,59례Ala/Ala형.Val379Ala기인형화등위기인빈솔:농독증조여대조조비교차이무통계학의의(P>0.05),존활조、사망조여대조조비교차이무통계학의의(P>0.05),경형농독증조、엄중농독증조여대조조비교차이무통계학의의(P>0.05).결론 PAF-AH기인Val379Ala위점다태성여농독증역감성、예후급병정엄중정도가능불상관.
Objective To discuss the allele frequency and genotypic distribution of platelet -activating factor acetylhydrolase ( PAF - AH ) gene polymorphism in the patients with sepsis. Methods Val379Ala site genotypes were determined in the patients (n = 82) and healthy controls (n=90) by means of restriction fragment length polymorphism analysis of polymerase chain reaction products, DNA sequencing was used to detect the PCR product containing allele gene polymorphism. SPSS 13.0 statisti-cal software was used to analyze. Results All the samples by PCR - RFLP analysis of the PAF-AH gene in the Val379Ala site had three kinds of genotypes; in 82 cases of sepsis group, there were 1 homo-zygous Val/Val type, 24 heterozygous Val/Ala type, 57 homozygous Ala/Ala type; in 90 cases of con-trol group, there were 2 Val/Val type, 29 Val/Ala type, 59 Ala/Ala type. The Val379Ala allele fre-quency and genotypic distribution in the patients with sepsis was not significantly different from those in the healthy controls ( P > 0. 05) ; no statistically significant difference was observed among the survival group, the death group and the control group ( P > 0. 05 ) ; no statistically significant difference was ob-served among the mild sepsis group, severe sepsis group and control group(P > 0. 05). Conclusion No correlations were found between PAF - AH gene Val379Ala polymorphism and sepsis.