CAJ | 학술논문

为探索小麦水通道蛋白在氮素处理过程中的生物学功能,以0.1%尿素处理6.0 h的周麦19幼根为材料,利用RT-PCR方法克隆了小麦PIPs类型的水通道蛋白基因TaPIP1,并分析了该基因的组织表达特性及其在尿素处理过程中的表达特征.TaPIP1全长1 062 bp,包括61 bp的5'非翻译区,128 bp的 3'非翻译区和一个长度为873 bp、编码290个氨基酸的开放阅读框.序列分析表明,TaPIP1基因与已知小麦(GQ452384和AAM00368)、大麦(BAA23746,CAA54233和BAA23745)、水稻(BAA24016)和玉米(AAK26754 ,ACG39699,ACG37183,CAA57955和AAK26756)等单子叶植物来源的同类基因同源性较高,相似性为85.9%～99.3%.半定量RT-PCR表达谱分析显示,TaPIP1在抽穗期小麦的根、茎和旗叶中均能表达.氮素处理下该基因表达分析结果显示,TaPIP1在萌发期小麦根系中的表达受尿素的诱导.
위탐색소맥수통도단백재담소처리과정중적생물학공능,이0.1%뇨소처리6.0 h적주맥19유근위재료,이용RT-PCR방법극륭료소맥PIPs류형적수통도단백기인TaPIP1,병분석료해기인적조직표체특성급기재뇨소처리과정중적표체특정.TaPIP1전장1 062 bp,포괄61 bp적5'비번역구,128 bp적 3'비번역구화일개장도위873 bp、편마290개안기산적개방열독광.서렬분석표명,TaPIP1기인여이지소맥(GQ452384화AAM00368)、대맥(BAA23746,CAA54233화BAA23745)、수도(BAA24016)화옥미(AAK26754 ,ACG39699,ACG37183,CAA57955화AAK26756)등단자협식물래원적동류기인동원성교고,상사성위85.9%～99.3%.반정량RT-PCR표체보분석현시,TaPIP1재추수기소맥적근、경화기협중균능표체.담소처리하해기인표체분석결과현시,TaPIP1재맹발기소맥근계중적표체수뇨소적유도.
In order to study the function of aquaporin proteins (water channels proteins) under urea treatment in Wheat, seedlings of wheat cultivar "Zhoumai 19" treated with urea solution (0.1 %) were prepared and a PIPs type gene TaPIP1 was cloned by RT-PCR , and its expression pattern was studied via semi-quantity RT-PCR. TaPIP1 was 1062 bp in full length, including 5' untranslated region of 61 bp, 3' untranslated region of 128 bp, and an open reading frame (ORF) encoding 290 amino acid. Homology analysis showed that the deduced amino acid sequence of TaPIP1 shared 85.9%～99.3% identity with aquaporins from monocotyledon, such as wheat (Triticum aestivum, GQ452384 and AAM00368), barley (Hordeum vulgare, BAA23746, CAA54233 and BAA23745), rice (Oriza sativa, BAA24016) and maize (Zea mays, AAK26754, ACG39699, ACG37183, CAA57955 and AAK26756). Semi-quantity RT-PCR analysis showed that the TaPIP1 was expressed in roots, stems and flag leaves. The expression profiling also showed that TaPIP1 expression in wheat roots was up-regulated by urea treatment.