CAJ | 학술논문

除血浆之外,尿液是另一种寻找潜在生物标志物的重要生物材料.本研究以200000×g超速离心法分离正常人和非小细胞肺癌(NSCLC)患者尿液中的Exosomes,运用1D SDS-PAGE对Exosomes蛋白质组进行分组,从电泳胶上切取正常组和疾病组的20～31 kDa条带,胰蛋白酶酶解后,进行HPLC-CHIP-MS/MS分析,并通过UniProtKB/SWISS-PORT数据库搜索鉴定了24种蛋白质,其中在NSCLC患者尿液Exosomes蛋白质组中发现了8种差异表达蛋白,包括免疫球蛋白κ的3个片段、2种Ras相关蛋白、谷胱甘肽S转移酶A2、血清淀粉样P成分前体和磷脂酰乙醇胺结合蛋白1.
제혈장지외,뇨액시령일충심조잠재생물표지물적중요생물재료.본연구이200000×g초속리심법분리정상인화비소세포폐암(NSCLC)환자뇨액중적Exosomes,운용1D SDS-PAGE대Exosomes단백질조진행분조,종전영효상절취정상조화질병조적20～31 kDa조대,이단백매매해후,진행HPLC-CHIP-MS/MS분석,병통과UniProtKB/SWISS-PORT수거고수색감정료24충단백질,기중재NSCLC환자뇨액Exosomes단백질조중발현료8충차이표체단백,포괄면역구단백κ적3개편단、2충Ras상관단백、곡광감태S전이매A2、혈청정분양P성분전체화린지선을순알결합단백1.
Urine provides an alternative to blood plasma as a potential source of disease biomarkers. Exosomes was separated by ultracentrifuge at 200000 g in normal persons and non-small cell lung cancer (NSCLC) patients′ urine. For proteomic analysis of urinary exosome, 1D sodium dodecylsulfonate-polyacrylate gel electrophoresis(SDS-PAGE) was carries out and cut the gel 31 kDa-20 kDa bands in normal group and disease group′s. These gel blocks were subjected to in-gel trypsinization, and the extracted peptides were analyzed HPLC-CHIP-MS/MS. Approximately 24 unique proteins were identified in the UniProtKB/SWISS-PORT. The difference expression proteins were found in urinary exosome from NSCLC patients, including three fragment of the immunoglobulin kappa, two kinds of Ras related proteins, glutathione S-transferase A2, serum amyloid P-component precursor and phosphatidylethanolamine-binding protein 1.