CAJ | 학술논문

目的应用蛋白质组学表面增强激光解吸电离-飞行时间-质谱( SELDI-TOF-MS)技术筛选淋巴瘤患者血清特异性生物标志物,发现淋巴瘤早期诊断与人群筛查的血清学特异性标志.方法应用SELDI-TOF-MS技术检测96例淋巴瘤患者血清特异性标志物,包括非霍奇金淋巴瘤(NHL) 86例,霍奇金淋巴瘤( HL) 10例,以30名健康体检者作为对照.采用生物化学法测定乳酸脱氢酶(LDH),酶联免疫吸附法( ELISA)测定可溶性白细胞介素-2受体(sIL-2R)、β2-微球蛋白(β2-MG)和糖链抗原125( CA125).结果在质荷比(M/Z)为0.2～16.0间,NHL患者有15个蛋白质含量与健康人差异有统计学意义(P＜0.01),其中M/Z为6880、8564、8692和13751的蛋白质低表达对淋巴瘤的敏感度与特异度高,灵敏度和特异度分别为82.29％和80.00％、78.13％和73.30％、75.00％和80.00％、71.88％和83.30％.4个蛋白质对淋巴瘤诊断的灵敏度与特异度差异均无统计学意义(P＞0.05),4个蛋白质联合检测淋巴瘤特异度达100.00％,与LDH的灵敏度21.74％、CA125的灵敏度56.80％、β2-MG的灵敏度70.50％比较,差异均有统计学意义(P＜0.05).结论对M/Z为6880、8564、8692和13751的蛋白质行质谱检测,有望作为人群筛查、诊断淋巴瘤的血清学特异性标志.
목적 응용단백질조학표면증강격광해흡전리-비행시간-질보( SELDI-TOF-MS)기술사선림파류환자혈청특이성생물표지물,발현림파류조기진단여인군사사적혈청학특이성표지.방법 응용SELDI-TOF-MS기술검측96례림파류환자혈청특이성표지물,포괄비곽기금림파류(NHL) 86례,곽기금림파류( HL) 10례,이30명건강체검자작위대조.채용생물화학법측정유산탈경매(LDH),매련면역흡부법( ELISA)측정가용성백세포개소-2수체(sIL-2R)、β2-미구단백(β2-MG)화당련항원125( CA125).결과 재질하비(M/Z)위0.2～16.0간,NHL환자유15개단백질함량여건강인차이유통계학의의(P＜0.01),기중M/Z위6880、8564、8692화13751적단백질저표체대림파류적민감도여특이도고,령민도화특이도분별위82.29％화80.00％、78.13％화73.30％、75.00％화80.00％、71.88％화83.30％.4개단백질대림파류진단적령민도여특이도차이균무통계학의의(P＞0.05),4개단백질연합검측림파류특이도체100.00％,여LDH적령민도21.74％、CA125적령민도56.80％、β2-MG적령민도70.50％비교,차이균유통계학의의(P＜0.05).결론 대M/Z위6880、8564、8692화13751적단백질행질보검측,유망작위인군사사、진단림파류적혈청학특이성표지.
Objective To identify serum specific protein biomarkers for the early diagnosis of lymphoma patients by using surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) protein chip array.Methods SELDI-TOF-MS technique and weak cation exchanger (WCX2) protein chip were used to detect the serum proteomic patterns of 96 patients with lymphoma (86 patients with non-Hodgkin lymphoma and 10 patients with Hodgkin lymphoma) and 30 normal control.The serum level of LDH were measured by biochemical methods,the serum level of β2-MG and CA125 were detected by enzyme-linked immunosorbent assay (ELISA).Results Four protein peaks pattern mass/charge ratio (M/Z) 6880,8564,8692 and 13751 were decreased in lymphoma patients,and they could be used to distinguish lymphoma from healthy people and other malignant tumors.Their sensitivity and specificity were 82.29 ％ and 80.00 ％,78.13 ％ and 73.30 ％,75.00 ％ and 80.00 ％,71.88 ％ and 83.30 ％ respectively in lymphoma patients.When it was used to early diagnosing lymphoma in stage Ⅰ or Ⅱ,the sensitivity of four protein peaks pattern (M/Z 6880,8564,8692 and 13 751) were 77.55 ％,71.43 ％,71.43 ％ and 67.35 ％respectively and the specificity of all four protein peaks pattern were 100.00 ％.Four protein peaks pattern sensitivity were higher than LDH,CA125 and β2-MG respectively.Conclusion Application of SELDI-TOF-MS can be of great potential for the early diagnosis of lymphoma patients.