CAJ | 학술논문

目的探讨离体供肝持续低温灌注对大鼠移植肝脏的保护作用.方法 60只Wistar大鼠随机分为3组,假手术组12只.离体供肝进行持续性冷灌注组和常规冷保存组各24只,供肝保存时间5 h.假手术组及后两组受体分别于术后24 h留取血液、肝组织,并记24 h平均胆汁流量.各组分别检测肝功能,测定胆汁中γ-谷氨酰转肽酶(GGT)活性及萄萄糖(Glu)含量.肝组织行常规病理学检查.结果持续低温灌注组和常规冷保存组血清肝功能指标均高于假手术组(P＜0.05),而持续低温灌注组较常规冷保存组低(P＜0.05).持续低温灌注组平均每小时胆汁流量明显高于冷保存组(P＜0.05).持续低温灌注组胆汁中GGT活性和Glu含量明显低于常规冷保存组(P＜0.05).结论离体供肝持续低温灌注对大鼠移植后肝功能有明显的保护作用.
목적 탐토리체공간지속저온관주대대서이식간장적보호작용.방법 60지Wistar대서수궤분위3조,가수술조12지.리체공간진행지속성랭관주조화상규랭보존조각24지,공간보존시간5 h.가수술조급후량조수체분별우술후24 h류취혈액、간조직,병기24 h평균담즙류량.각조분별검측간공능,측정담즙중γ-곡안선전태매(GGT)활성급도도당(Glu)함량.간조직행상규병이학검사.결과 지속저온관주조화상규랭보존조혈청간공능지표균고우가수술조(P＜0.05),이지속저온관주조교상규랭보존조저(P＜0.05).지속저온관주조평균매소시담즙류량명현고우랭보존조(P＜0.05).지속저온관주조담즙중GGT활성화Glu함량명현저우상규랭보존조(P＜0.05).결론 리체공간지속저온관주대대서이식후간공능유명현적보호작용.
Objective To explore the protective effect of continuous hypothermic perfusion on donor liver. Methods Sixty Wistar rats were randomly divided into the control group (sham operation group, n＝12), donor continuous hypothermic reperfusion group (n＝24) and static cold storage group (n＝24). The donor keeping time was 5 h. The samples of blood and hepatic tissue were col-lected 24 h after liver transplantation. Meanwhile, the bile flow during the 24 h was recorded. Results Compared with the control group, liver functional parameters were higher in other two groups (P＜0.05). It was significantly lower in the continuous hypothermic reperfusion group than in the static cold storage group (P＜0.05). Meanwhile, the bile flow in the continuous hypothermic reperfusion group was more than that in the static cold storage group (P＜0.05). While γ-GGT and the Glucose(Glu) in the bile of the continuous hypothermic reperfusion group were lower than those in the static cold storage group (P＜0.05). Conclusions Continuous hypothermic perusion of donor liver during the keeping time has protective effect on the liver after transplantation.