CAJ | 학술논문

目的研究家族成员中反复腹痛和无腹痛者的幽门螺杆菌(Helicobacter priori,Hp)感染和人类白细胞抗原(HLA)-DQA1等位基因频率分布.方法将反复腹痛患儿为核心的20个家庭118名同代、一级和二级亲属分为反复腹痛组和无腹痛组,应用胶体金标免疫渗滤法和免疫印迹法检测118名家族成员血Hp-IgG抗体和亚型.用聚合酶链反应-序列特异性引物(PCR-SSP)方法对2组进行HLA-DQA1等位基因分型.样本的分布经过Hardy-Weinberg平衡检验,P>0.05,组间HLA-DQA1等位基因比较采用四格表X2检验.结果 20个家族中Hp抗体和Hp亚型阳性率分别为100%和96.6%,Ⅰ型Hp感染占55.1%(65/118),Ⅱ型Hp感染为41.5%(49/118).反复腹痛组HLADQA1*0302的等位基因频率明显高于无腹痛组,2组比较差异有统计学意义(23%vs.2%,X2=13.277,P=0.000).结论在Hp感染的家族成员中,反复腹痛者与无腹痛者之间存在免疫遗传学差异,HLA-DQA1*0302可能是Hp感染后反复腹痛的相关基因.
목적 연구가족성원중반복복통화무복통자적유문라간균(Helicobacter priori,Hp)감염화인류백세포항원(HLA)-DQA1등위기인빈솔분포.방법 장반복복통환인위핵심적20개가정118명동대、일급화이급친속분위반복복통조화무복통조,응용효체금표면역삼려법화면역인적법검측118명가족성원혈Hp-IgG항체화아형.용취합매련반응-서렬특이성인물(PCR-SSP)방법대2조진행HLA-DQA1등위기인분형.양본적분포경과Hardy-Weinberg평형검험,P>0.05,조간HLA-DQA1등위기인비교채용사격표X2검험.결과 20개가족중Hp항체화Hp아형양성솔분별위100%화96.6%,Ⅰ형Hp감염점55.1%(65/118),Ⅱ형Hp감염위41.5%(49/118).반복복통조HLADQA1*0302적등위기인빈솔명현고우무복통조,2조비교차이유통계학의의(23%vs.2%,X2=13.277,P=0.000).결론 재Hp감염적가족성원중,반복복통자여무복통자지간존재면역유전학차이,HLA-DQA1*0302가능시Hp감염후반복복통적상관기인.
Objective To investigate Helicobacter pylori(Hp)infection and HLA-DQA1 allelic frequency in family members of children with recurrent abdominal pain.Methods One hundred and eighteen family members of 20 children with recurrent abdominal pain were divided into two groups:with and without recurrent abdominal pain.Serum Hp antibody was tested by dot immunogold filtration assay and immunophenotyping was determined by Western blot(immunobiot)technique.Polymerase chain reactionsequence specific primers(PCR-SSP)technique Was applied to identify HLA-DQAi allelic frequencies.Hardy-Weinberg equilibrium test was performed(P>0.05),and Chi-square test was used to compare the frequency of HLA-DQA1 alleles between the groups.Results The Hp seropositive rate in 118 members Was 100%and the Hp immunophenotyping was 96.6%.The prevalence of Hp Ⅰ and Ⅱ type was 55.1%(65/118)and41.5%(49/118).HLA-DQA1*0302 allelic frequency Was significantly higher in subjects with recurrent abdominal pain than that in subjects without one(23%vs.2%,X2=13.277,P=0.000).Conclusion There is immunogenetic difference between familial members with and without recurrent abdominal pain infected by Hp,and HLA-DQA1*0302 may be the associated gene contributing to different clinical outcomes after Hp infections.