CAJ | 학술논문

目的探讨氢气(hydrogen,H2)干预对心搏骤停后脑损伤的作用.方法实验在厦门大学动物实验中心进行,新西兰白兔60只,随机(随机数字法)分为2组:A组为2％氢气干预,B组为空气对照.比较血清中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、神经元特异性烯醇化酶( neuron-specific enolase,NSE)、S100β蛋白质量浓度及神经功能缺损评分(neurological deficit scores,NDS)变化,分析全身炎症反应综合征(systemic inflammatory response syndrome,SIRS)诊断数和兔存活数的差异.结果 A、B两组兔自主循环恢复(restoration of spontaneous circulation,ROSC)数量接近,随着时间延长,存活兔减少,到ROSC 72 h时,A组兔存活数高于B组(x2=4.850,P＜0.05).心肺复苏(cardiopulmonary resuscitation,CPR)后B组血中H2质量浓度于ROSC 4 h达峰值后下降；A组H2浓度保持在较高水平上.在ROSC后,两组兔均有不同程度炎症反应发生,ROSC 24 h时,A组TNF-α质量浓度、SIRS诊断数低于B组(P＜0.05).两组血清NSE和S100β质量浓度在CPR后升高,分别于ROSC 24 h和4h达峰值后下降,A组峰值低于B组(P＜0.05).两组兔在CPR后NDS分值逐渐下降,ROSC 72 h时,A组NDS低于B组(t=-2.689,P=0.013).结论 H2能抑制CPR后炎症反应,减轻心搏骤停后脑损伤.
목적 탐토경기(hydrogen,H2)간예대심박취정후뇌손상적작용.방법 실험재하문대학동물실험중심진행,신서란백토60지,수궤(수궤수자법)분위2조:A조위2％경기간예,B조위공기대조.비교혈청중종류배사인자-α(tumor necrosis factor-α,TNF-α)、신경원특이성희순화매( neuron-specific enolase,NSE)、S100β단백질량농도급신경공능결손평분(neurological deficit scores,NDS)변화,분석전신염증반응종합정(systemic inflammatory response syndrome,SIRS)진단수화토존활수적차이.결과 A、B량조토자주순배회복(restoration of spontaneous circulation,ROSC)수량접근,수착시간연장,존활토감소,도ROSC 72 h시,A조토존활수고우B조(x2=4.850,P＜0.05).심폐복소(cardiopulmonary resuscitation,CPR)후B조혈중H2질량농도우ROSC 4 h체봉치후하강；A조H2농도보지재교고수평상.재ROSC후,량조토균유불동정도염증반응발생,ROSC 24 h시,A조TNF-α질량농도、SIRS진단수저우B조(P＜0.05).량조혈청NSE화S100β질량농도재CPR후승고,분별우ROSC 24 h화4h체봉치후하강,A조봉치저우B조(P＜0.05).량조토재CPR후NDS분치축점하강,ROSC 72 h시,A조NDS저우B조(t=-2.689,P=0.013).결론 H2능억제CPR후염증반응,감경심박취정후뇌손상.
Objective To investigate the effects of hydrogen on post - cardiac arrest brain injury in rabbits.Method Sixty New Zealand rabbits were randomly divided into two groups,namely experiment group ( group A,n =30 ) and control group ( group B,n =30 ).Inhalation of 2％ hydrogen gas was conferred to rabbits immediately at the end of cardiac arrest modeling for 72 hours in the group A. Air instead was given to rabbits in the group B.Blood samples were collected before cardiac arrest (CA),and 4,12,24 and 72 hours after restoration of spontaneous circulation (ROSC) in all rabbits for determining the levels of hydrogen,tumor necrosis factor - α ( TNF - α),neuron - specific enolase (NSE) and protein S100β.At the same time,rectal temperature,mean arterial pressure,heart rate and respiration rate were recorded,and the neurologic deficit scoring (NDS) was carried out.The rate of systemic inflammatory response syndrome ( SIRS ) and the rate of survival of rabbits were analyzed. Results There was no significant difference in level of TNF - α activation between group A and group B within12 h of cardiopulmonary resuscitation (CPR).In group A,TNF - α level and the rate of SIRS peaked at 24 hours after CPR,which were higher than those in group B,and then decreased gradually,and the rate of survival was higher than that in group B in 72 hours after ROSC,the NSE was lower than that in group B at 24 hours after ROSC.In group B,S100β level began to increase significantly 4 hours after CPR,which was higher than that in group A,the level of NDS in group B was higher than that in group A 72 hours after ROSC.Conclusions Inhalation of hydrogen gas lessened inflammation and alleviated the brain injury after CPR.