中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2010年
11期
766-769
,共4页
邓列华%李璟蓉%胡云峰%许向前%吴焱%徐瑾%郭秀枝%刘洁%范洪涛%林泽%赵永铿
鄧列華%李璟蓉%鬍雲峰%許嚮前%吳焱%徐瑾%郭秀枝%劉潔%範洪濤%林澤%趙永鏗
산렬화%리경용%호운봉%허향전%오염%서근%곽수지%류길%범홍도%림택%조영갱
尖锐湿疣%生存素%环氧合酶2%血管内皮生长因子
尖銳濕疣%生存素%環氧閤酶2%血管內皮生長因子
첨예습우%생존소%배양합매2%혈관내피생장인자
Condyloma acuminata%Survivin%Cyclooxygenase 2%Vascular endothelial growth factor
目的 探讨生存素、环氧合酶-2(COX-2)、血管内皮生长因子(VEGF)在尖锐湿疣组织中的表达及其与尖锐湿疣血管生成的关系.方法 应用免疫组化PowerVision法对60例尖锐湿疣组织、21例正常包皮组织中生存素、COX-2、VEGF蛋白进行检测,根据CD105阳性的血管内皮细胞计数来测定尖锐湿疣组织微血管密度(MVD).结果 生存素在尖锐湿疣组织中阳性表达率为56.67%,正常包皮组织中为9.52%;COX-2在尖锐湿疣组织中阳性表达率为63.33%,正常包皮组织中为0;两组比较,差异均有统计学意义(P值均<0.05).生存素、COX-2在两组的阳性表达强度比较差异均有统计学意义(P值均<0.05).VEGF在尖锐湿疣组织、正常包皮组织中阳性表达率均为100%,但两组阳性表达强度比较差异有统计学意义(P<0.05).尖锐湿疣组织的MVD值(16.38±5.46)明显高于正常包皮组(0.62±0.44),差异有统计学意义(P<0.05).在尖锐湿疣组织中,生存素、COX-2与VEGF蛋白表达两两之间,生存素与MVD,COX-2与MVD均呈显著正相关.结论 生存素、COX-2、VEGF在尖锐湿疣组织中表达程度均高于对照组.
目的 探討生存素、環氧閤酶-2(COX-2)、血管內皮生長因子(VEGF)在尖銳濕疣組織中的錶達及其與尖銳濕疣血管生成的關繫.方法 應用免疫組化PowerVision法對60例尖銳濕疣組織、21例正常包皮組織中生存素、COX-2、VEGF蛋白進行檢測,根據CD105暘性的血管內皮細胞計數來測定尖銳濕疣組織微血管密度(MVD).結果 生存素在尖銳濕疣組織中暘性錶達率為56.67%,正常包皮組織中為9.52%;COX-2在尖銳濕疣組織中暘性錶達率為63.33%,正常包皮組織中為0;兩組比較,差異均有統計學意義(P值均<0.05).生存素、COX-2在兩組的暘性錶達彊度比較差異均有統計學意義(P值均<0.05).VEGF在尖銳濕疣組織、正常包皮組織中暘性錶達率均為100%,但兩組暘性錶達彊度比較差異有統計學意義(P<0.05).尖銳濕疣組織的MVD值(16.38±5.46)明顯高于正常包皮組(0.62±0.44),差異有統計學意義(P<0.05).在尖銳濕疣組織中,生存素、COX-2與VEGF蛋白錶達兩兩之間,生存素與MVD,COX-2與MVD均呈顯著正相關.結論 生存素、COX-2、VEGF在尖銳濕疣組織中錶達程度均高于對照組.
목적 탐토생존소、배양합매-2(COX-2)、혈관내피생장인자(VEGF)재첨예습우조직중적표체급기여첨예습우혈관생성적관계.방법 응용면역조화PowerVision법대60례첨예습우조직、21례정상포피조직중생존소、COX-2、VEGF단백진행검측,근거CD105양성적혈관내피세포계수래측정첨예습우조직미혈관밀도(MVD).결과 생존소재첨예습우조직중양성표체솔위56.67%,정상포피조직중위9.52%;COX-2재첨예습우조직중양성표체솔위63.33%,정상포피조직중위0;량조비교,차이균유통계학의의(P치균<0.05).생존소、COX-2재량조적양성표체강도비교차이균유통계학의의(P치균<0.05).VEGF재첨예습우조직、정상포피조직중양성표체솔균위100%,단량조양성표체강도비교차이유통계학의의(P<0.05).첨예습우조직적MVD치(16.38±5.46)명현고우정상포피조(0.62±0.44),차이유통계학의의(P<0.05).재첨예습우조직중,생존소、COX-2여VEGF단백표체량량지간,생존소여MVD,COX-2여MVD균정현저정상관.결론 생존소、COX-2、VEGF재첨예습우조직중표체정도균고우대조조.
Objective To investigate the expressions of survivin, cyclooxyenase-2 (COX-2) and vascular endothelial growth factor (VEGF) and their relationship with angiogenesis in condyloma acuminatum (CA) tissues. Methods Immunohistochemistry using PowerVision staining kit was performed to detect the expression of survivin, COX-2 and VEGF protein in 60 CA tissue samples from patients and 21 normal skin samples from the foreskin of human controls. At the same time, the microvessel density was determined in CA tissues by staining blood vessel endothelium with anti-CD105 monoclonal antibody. Results The positivity rate of survivin and COX-2 expression was 56.67% and 63.33%, in CA tissues, 9.52% and 0 in normal skin tissues, respectively. There was a significant difference between the two groups of tissue samples in the positivity rate and intensity of survivin and COX-2 expression (all P < 0.05). VEGF was expressed in all of the CA tissues and normal skin tissues, while the intensity of VEGF expression was statistically different between the two groups of tissue samples (P < 0.05). The MVD was 16.38 ± 5.46 and 0.62 ± 0.44 in CA tissues and normal skin tissues, respectively (P < 0.05). There was a significant positive correlation between the expressions of survivin, COX-2 and VEGF, as well as between MVD and the expressions of survivin and COX-2 in CA tissues. Conclusion The expression levels of survivin, COX-2 and VEGF are significantly higher in CA tissues than in normal skin tissues.
