中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2009年
3期
297-300
,共4页
周家鹏%杨竹林%刘栋才%周建平
週傢鵬%楊竹林%劉棟纔%週建平
주가붕%양죽림%류동재%주건평
胃肿瘤%胃炎%胃溃疡%胃息肉%半乳糖凝集素3%接骨木凝集素
胃腫瘤%胃炎%胃潰瘍%胃息肉%半乳糖凝集素3%接骨木凝集素
위종류%위염%위궤양%위식육%반유당응집소3%접골목응집소
Stomach neoplasms%Gastritis%Stomach ulcer%Gastric polyp%Galectin-3%Sambucus nigra agglutitin
目的 研究胃良性和恶性病变组织中β-半乳糖凝集素-3(gal-3)和接骨木凝集素(SNA)的表达水平及其临床病理意义.方法 将49例胃癌、20例癌旁组织、36例淋巴结转移灶和80例不同类型胃良性病变手术切除或胃镜活检标本常规制作石蜡包埋切片,gal-3染色方法为EnVisionTM免疫组织化学(免疫组化)法,SNA染色方法为ABC亲和组化法.结果 胃癌组织gal-3和SNA表达阳性率明显高于癌旁组织及不同类型胃良性病变者(P<0.05,P<0.01),阳性表达的胃良性病变标本和癌旁组织均呈不同程度不典型增生;淋巴结转移灶gal-3和SNA阳性率分别为63.9%和55.6%,与其相应的原发病灶gal-3和SNA阳性率(72.2%和66.7%)比较.差异无统计学意义(P>0.05).组织学分级Ⅱ级、浸润深度T1和T2及无区域淋巴结转移的胃癌组织gal-3和SNA表达阳性率明显低于组织学分级Ⅲ、Ⅳ级和浸润深度T1、T4及区域淋巴结转移者(P<0.05):淋巴结转移N1及无远处器官转移胃癌组织gal-3和SNA表达阳性率低于淋巴结转移N2、N3站及远处器官转移的胃癌组织,但差异均无统计学意义(P>0.05);胃癌组织中,31例gal-3表达阳性病例中SNA阳性22例,两者表达呈明显一致性(χ2=6.59,P<0.05).结论 gal-3和SNA表达水平可能是反映胃癌发生、进展及其临床生物学行为的重要凝集素分子标记物.
目的 研究胃良性和噁性病變組織中β-半乳糖凝集素-3(gal-3)和接骨木凝集素(SNA)的錶達水平及其臨床病理意義.方法 將49例胃癌、20例癌徬組織、36例淋巴結轉移竈和80例不同類型胃良性病變手術切除或胃鏡活檢標本常規製作石蠟包埋切片,gal-3染色方法為EnVisionTM免疫組織化學(免疫組化)法,SNA染色方法為ABC親和組化法.結果 胃癌組織gal-3和SNA錶達暘性率明顯高于癌徬組織及不同類型胃良性病變者(P<0.05,P<0.01),暘性錶達的胃良性病變標本和癌徬組織均呈不同程度不典型增生;淋巴結轉移竈gal-3和SNA暘性率分彆為63.9%和55.6%,與其相應的原髮病竈gal-3和SNA暘性率(72.2%和66.7%)比較.差異無統計學意義(P>0.05).組織學分級Ⅱ級、浸潤深度T1和T2及無區域淋巴結轉移的胃癌組織gal-3和SNA錶達暘性率明顯低于組織學分級Ⅲ、Ⅳ級和浸潤深度T1、T4及區域淋巴結轉移者(P<0.05):淋巴結轉移N1及無遠處器官轉移胃癌組織gal-3和SNA錶達暘性率低于淋巴結轉移N2、N3站及遠處器官轉移的胃癌組織,但差異均無統計學意義(P>0.05);胃癌組織中,31例gal-3錶達暘性病例中SNA暘性22例,兩者錶達呈明顯一緻性(χ2=6.59,P<0.05).結論 gal-3和SNA錶達水平可能是反映胃癌髮生、進展及其臨床生物學行為的重要凝集素分子標記物.
목적 연구위량성화악성병변조직중β-반유당응집소-3(gal-3)화접골목응집소(SNA)적표체수평급기림상병리의의.방법 장49례위암、20례암방조직、36례림파결전이조화80례불동류형위량성병변수술절제혹위경활검표본상규제작석사포매절편,gal-3염색방법위EnVisionTM면역조직화학(면역조화)법,SNA염색방법위ABC친화조화법.결과 위암조직gal-3화SNA표체양성솔명현고우암방조직급불동류형위량성병변자(P<0.05,P<0.01),양성표체적위량성병변표본화암방조직균정불동정도불전형증생;림파결전이조gal-3화SNA양성솔분별위63.9%화55.6%,여기상응적원발병조gal-3화SNA양성솔(72.2%화66.7%)비교.차이무통계학의의(P>0.05).조직학분급Ⅱ급、침윤심도T1화T2급무구역림파결전이적위암조직gal-3화SNA표체양성솔명현저우조직학분급Ⅲ、Ⅳ급화침윤심도T1、T4급구역림파결전이자(P<0.05):림파결전이N1급무원처기관전이위암조직gal-3화SNA표체양성솔저우림파결전이N2、N3참급원처기관전이적위암조직,단차이균무통계학의의(P>0.05);위암조직중,31례gal-3표체양성병례중SNA양성22례,량자표체정명현일치성(χ2=6.59,P<0.05).결론 gal-3화SNA표체수평가능시반영위암발생、진전급기림상생물학행위적중요응집소분자표기물.
Objective To study the expressive levels of galectin-3(gal-3) and sambucus nigra agglutinin (SNA) and their clinicopathological significance in the benign and malignant lesions of stomach. Methods EnVisonTM immunohistochemistry for assaying gal-3 expressive level and ABC cytochemistry for determining SNA expressive level were used in conventional paraffin-embedded sections from specimens of gastric cancer(n=49), peritumoral tissues(n=20), metastastic foci of lymph nodes(n=36), and different types of benign lesions(n=80). Results The positive rates of gal-3 and SNA were significantly higher in gastric cancer tissues than those in peritumoral tissues and different types of benign lesions (P<0.05, P<0.01). The positive cases of gal-3 and/or SNA in peritumoral tissues and benign lesions showed mild- to severe-atypical hyperplasia of mucous epithelial cells. No difference was found between the primary foci and metastatic foci in gal-3 and SNA expressions (P>0.05). The positive rates of gal-3 and SNA were significantly lower in histologic grade Ⅱ, infitrating depth T1,T2 and no-metastasis of regional lymph node than those in histologic grade Ⅲ, Ⅳ, infitrating depth T3,T4 and metastasis of lymph node in gastric cancer(P<0.05). The positive rates of gal-3 and SNA were higher in lymphnede metastatic site N1 and no-metastasis of distant organs than those in lymphnede metastatic site N2, N3 and metastasis of distant organs, but no significant difference was found (P>0.05). The consistence was found between the expression of gal-3 and SNA in gastric cancer tissues (χ2=6.59,P<0.05). Conclusions The expressive levels of gal-3 and SNA may be important molecular markers of lectins for reflecting the carcinogenesis, progression and biological behaviors in gastric cancer.
