中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2011年
4期
293-298
,共6页
刘少锋%葛平江%张思毅%汪斌超%祁周措%盛晓丽
劉少鋒%葛平江%張思毅%汪斌超%祁週措%盛曉麗
류소봉%갈평강%장사의%왕빈초%기주조%성효려
喉肌%卫星细胞,骨骼肌%喉返神经%声带麻痹%狗
喉肌%衛星細胞,骨骼肌%喉返神經%聲帶痳痺%狗
후기%위성세포,골격기%후반신경%성대마비%구
Laryngeal muscles%Satellite cells,skeletal muscle%Recurrent laryngeal nerve%Vocal cord paralysis%Dogs
目的 探讨家犬双侧环杓后肌失神经支配及用颈袢神经再吻合支配后肌卫星细胞活性的变化.方法 随机数字表法将24只家犬分成3组,每组8只,分别为切断双侧喉返神经组,切断喉返神经后即刻颈袢神经再吻合组,不切断喉返神经的对照组.3组动物手术后再饲养9周后再次暴露喉返神经及环杓后肌,使用喉返神经诱发环杓后肌电位来验证喉返神经的再通情况;双盲法提取环杓后肌肌肉组织中总RNA,反转录后做实时定量聚合酶链反应(PCR)检测肌卫星细胞增殖分化标记物Myogenin、Myf5和Pax7的mRNA表达.结果 手术后3组动物各有1只死亡或感染退出实验.术后9周诱发神经环杓后肌肌电图提示对照组动物喉返神经功能均正常,切断组7只动物电刺激都没有反应,吻合组均有神经再通.Myogenin的mRNA相对表达量切断组较对照组和吻合组均有明显升高(Z值为1.42和1.38,P值均＜0.05),Myf5的mRNA表达切断组也明显高于对照组和吻合组(Z值为1.66和1.69,P值均＜0.01);切断组Pax7的mRNA表达明显高于对照组(Z=1.66,P＜0.01),也高于吻合组(Z=1.42,P＜0.05);而吻合组与对照组Myogenin、Myf5和Pax7的mRNA表达差异均无统计学意义(P值均＞0.05).结论 双侧喉返神经切断后,环杓后肌的肌卫星细胞增殖分化的mRNA表达增强,而同颈袢神经再吻合后肌卫星细胞的增殖分化表达下降.
目的 探討傢犬雙側環杓後肌失神經支配及用頸袢神經再吻閤支配後肌衛星細胞活性的變化.方法 隨機數字錶法將24隻傢犬分成3組,每組8隻,分彆為切斷雙側喉返神經組,切斷喉返神經後即刻頸袢神經再吻閤組,不切斷喉返神經的對照組.3組動物手術後再飼養9週後再次暴露喉返神經及環杓後肌,使用喉返神經誘髮環杓後肌電位來驗證喉返神經的再通情況;雙盲法提取環杓後肌肌肉組織中總RNA,反轉錄後做實時定量聚閤酶鏈反應(PCR)檢測肌衛星細胞增殖分化標記物Myogenin、Myf5和Pax7的mRNA錶達.結果 手術後3組動物各有1隻死亡或感染退齣實驗.術後9週誘髮神經環杓後肌肌電圖提示對照組動物喉返神經功能均正常,切斷組7隻動物電刺激都沒有反應,吻閤組均有神經再通.Myogenin的mRNA相對錶達量切斷組較對照組和吻閤組均有明顯升高(Z值為1.42和1.38,P值均＜0.05),Myf5的mRNA錶達切斷組也明顯高于對照組和吻閤組(Z值為1.66和1.69,P值均＜0.01);切斷組Pax7的mRNA錶達明顯高于對照組(Z=1.66,P＜0.01),也高于吻閤組(Z=1.42,P＜0.05);而吻閤組與對照組Myogenin、Myf5和Pax7的mRNA錶達差異均無統計學意義(P值均＞0.05).結論 雙側喉返神經切斷後,環杓後肌的肌衛星細胞增殖分化的mRNA錶達增彊,而同頸袢神經再吻閤後肌衛星細胞的增殖分化錶達下降.
목적 탐토가견쌍측배표후기실신경지배급용경번신경재문합지배후기위성세포활성적변화.방법 수궤수자표법장24지가견분성3조,매조8지,분별위절단쌍측후반신경조,절단후반신경후즉각경번신경재문합조,불절단후반신경적대조조.3조동물수술후재사양9주후재차폭로후반신경급배표후기,사용후반신경유발배표후기전위래험증후반신경적재통정황;쌍맹법제취배표후기기육조직중총RNA,반전록후주실시정량취합매련반응(PCR)검측기위성세포증식분화표기물Myogenin、Myf5화Pax7적mRNA표체.결과 수술후3조동물각유1지사망혹감염퇴출실험.술후9주유발신경배표후기기전도제시대조조동물후반신경공능균정상,절단조7지동물전자격도몰유반응,문합조균유신경재통.Myogenin적mRNA상대표체량절단조교대조조화문합조균유명현승고(Z치위1.42화1.38,P치균＜0.05),Myf5적mRNA표체절단조야명현고우대조조화문합조(Z치위1.66화1.69,P치균＜0.01);절단조Pax7적mRNA표체명현고우대조조(Z=1.66,P＜0.01),야고우문합조(Z=1.42,P＜0.05);이문합조여대조조Myogenin、Myf5화Pax7적mRNA표체차이균무통계학의의(P치균＞0.05).결론 쌍측후반신경절단후,배표후기적기위성세포증식분화적mRNA표체증강,이동경번신경재문합후기위성세포적증식분화표체하강.
Objective To investigate the activity of bilateral posterior cricoarytenoid muscle satellite cell after denervation or reinnervation with ansa cervicalis. Methods Twenty four dogs were randomly divided into 3 groups. The bilateral laryngeal recurrent nerves were cut in group one in all dogs. The bilateral laryngeal recurrent nerves were anastomosed with ansa cervicalis after incision in group two in all dogs. The dogs in group three were used as control. Nine weeks after surgery, the electromyography was used to test the regeneration of the nerve. The posterior cricoarytenoid muscles biopsy were collected. The expression of mRNA of Myogenin, Myf5, and Pax7 was assayed by realtime RT-PCR after total RNA isolation. Results Two dogs died after surgery in incision and anastomose group. The electromyography suggested that the RLN of all dogs had denervated in the incision group and had reinnervated in the anastomose group after 9 weeks. Myogenin mRNA from RLN incision dogs PCA muscles had greater expression versus controls ( Z = 1.42, P ＜ 0. 01 ) or anastomosed dogs ( Z = 1.38, P ＜ 0. 01 ). Myf5 mRNA expression from RLN incision dogs PCA muscles had significant increase versus control dogs ( Z = 1.66, P ＜0. 01 ) or anastomosed dogs ( Z = 1.69, P ＜ 0. 01 ). Pax7 mRNA expression from RNL incision dogs had significant increase compared with control ( Z = 1.66, P ＜0. 01 ) or anastomosed animals ( Z = 1.42, P ＜0. 05 ). There was no significant difference in Myogenin ( Z = 1.34, P ＞ 0. 05 ), Myf5 ( Z = 0. 54, P ＞0. 05) and Pax ( Z = 0. 54, P ＞ 0. 05 ) mRNA expression between controls and anastomosed animals.Conclusions The bilateral denervation of RLN cause significantly increasing in dog PCA muscle satellite cell proliferation and differentiation. The bilateral reinnervation of RLN cause PCA muscle satellite cell come back nonproliferative, quiescent state in dog.
