中华外科杂志
中華外科雜誌
중화외과잡지
CHINESE JOURNAL OF SURGERY
2010年
12期
929-932
,共4页
翟弘峰%徐林刚%郭再兰%邱长虹
翟弘峰%徐林剛%郭再蘭%邱長虹
적홍봉%서림강%곽재란%구장홍
细胞培养技术%转化生长因子%尿道狭窄%结缔组织生长因子
細胞培養技術%轉化生長因子%尿道狹窄%結締組織生長因子
세포배양기술%전화생장인자%뇨도협착%결체조직생장인자
Cell culture techniques%Transforming growth factors%Urethral stricture%Connective tissue growth factor
目的 观察转化生长因子β1(TGF-β1)对尿道黏膜上皮细胞及成纤维细胞的生长调节作用及在诱导靶细胞结缔组织生长因子(CTGF)的表达中的作用.方法 体外培养尿道黏膜上皮细胞及成纤维细胞并鉴定.取第4代细胞分别设立对照组(以不含TGF-β1的细胞培养液培养)和实验组(分别以TGF-β11、2、4、8 μg/L的细胞培养液培养),24 h后分别以MTT比色试验和细胞计数法检测细胞活力,RT-PCR检测细胞内CTGF mRNA的表达.结果 与对照组相比较,实验组尿道黏膜上皮细胞数量和吸光度值随TGF-β1浓度升高而降低(P＜0.05),成纤维细胞数量和吸光度值随TGF-βl浓度升高而升高(P＜0.05);CTGF mRNA在实验组上皮细胞和成纤维细胞中均有表达,且表达水平随TGF-βl浓度升高而增加(P＜0.05).结论 TGF-β1可抑制尿道黏膜上皮细胞生长,促进成纤维细胞生长,并能诱导尿道黏膜上皮细胞及成纤维细胞表达CTGF mRNA.
目的 觀察轉化生長因子β1(TGF-β1)對尿道黏膜上皮細胞及成纖維細胞的生長調節作用及在誘導靶細胞結締組織生長因子(CTGF)的錶達中的作用.方法 體外培養尿道黏膜上皮細胞及成纖維細胞併鑒定.取第4代細胞分彆設立對照組(以不含TGF-β1的細胞培養液培養)和實驗組(分彆以TGF-β11、2、4、8 μg/L的細胞培養液培養),24 h後分彆以MTT比色試驗和細胞計數法檢測細胞活力,RT-PCR檢測細胞內CTGF mRNA的錶達.結果 與對照組相比較,實驗組尿道黏膜上皮細胞數量和吸光度值隨TGF-β1濃度升高而降低(P＜0.05),成纖維細胞數量和吸光度值隨TGF-βl濃度升高而升高(P＜0.05);CTGF mRNA在實驗組上皮細胞和成纖維細胞中均有錶達,且錶達水平隨TGF-βl濃度升高而增加(P＜0.05).結論 TGF-β1可抑製尿道黏膜上皮細胞生長,促進成纖維細胞生長,併能誘導尿道黏膜上皮細胞及成纖維細胞錶達CTGF mRNA.
목적 관찰전화생장인자β1(TGF-β1)대뇨도점막상피세포급성섬유세포적생장조절작용급재유도파세포결체조직생장인자(CTGF)적표체중적작용.방법 체외배양뇨도점막상피세포급성섬유세포병감정.취제4대세포분별설립대조조(이불함TGF-β1적세포배양액배양)화실험조(분별이TGF-β11、2、4、8 μg/L적세포배양액배양),24 h후분별이MTT비색시험화세포계수법검측세포활력,RT-PCR검측세포내CTGF mRNA적표체.결과 여대조조상비교,실험조뇨도점막상피세포수량화흡광도치수TGF-β1농도승고이강저(P＜0.05),성섬유세포수량화흡광도치수TGF-βl농도승고이승고(P＜0.05);CTGF mRNA재실험조상피세포화성섬유세포중균유표체,차표체수평수TGF-βl농도승고이증가(P＜0.05).결론 TGF-β1가억제뇨도점막상피세포생장,촉진성섬유세포생장,병능유도뇨도점막상피세포급성섬유세포표체CTGF mRNA.
Objective To investigate the effects of transforming growth factor-βl (TGF-β1) on growth controling and the expression of connective tissue growth factor mRNA (CTGF mRNA) in urethra epithelium cells and fibroblasts cultured in vitro. Methods Urethra epithelial cells and fibroblasts were culturedin vitro and identified. The fourth generation cells were divided into control group( cultured by cell medium without TGF-βl ) and experimental groups( cultured by cell medium containing TGF-β1 1,2, 4 and 8 μg/L), the vital force of cells were examined by MTT and cell counting, the expression of CTGF mRNA were examined by RT-PCR after 24 hours. Results The optical density and cell count decreased in experimental groups of urethra epithelium cells and increased in experimental groups of fibroblasts with the concentration of TGF-β1 being heightened compared with the control group(P＜0.05 ). The expression of CTGF mRNA increased with the heightening concentration of TGF-β1 in all experimental groups of urethra epithelium cells and fibroblasts by RT-PCR (P ＜ 0. 05 ). Conclusions TGF-β1 can inhibit the growth of urethra epithelium cells and promote the growth of fibroblastsin vitro, it can induce the expression of CTGF mRNA in two cells above-mentioned.
