CAJ | 학술논문

目的探讨母鼠孕期和哺乳期补充二十二碳六烯酸(DHA)对高氧或空气复苏的窒息新生大鼠体内氧化和抗氧化水平的影响.方法孕鼠从妊娠第1天起随机给予不同饮食,从而分为普食组和DHA组(除普通饮食外每日给予100 mg DHA).新生鼠出生24 h内进行缺氧实验2 h,然后随机进入高氧复苏组和空气复苏组.实验共分4组:普食+高氧复苏组、普食+空气复苏组、DHA+高氧复苏组和DHA+空气复苏组.用分光光度法分别检测窒息后即刻、高氧1 d、3 d和7 d时新生鼠血浆中超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)水平.结果 DHA+高氧复苏组新生鼠在高氧1 d、3 d和7 d时的s0D水平分别为(93.7±16.8)U/ml、(128.2±11.5)U/ml和(170.9±9.0)U/ml,显著高于DHA+空气复苏组[(73.7±21.5)U/ml、(112.2±19.9)U/ml和(124.2±24.6)U/ml],差异均有统计学意义(P均<0.05).DHA+高氧复苏组新生鼠的MDA含量在高氧3 d和7 d时分别为(6.0±0.5)nmol/ml和(3.5±0.9)nmol/ml,均显著低于普食+高氧复苏组[分别为(7.5±1.1)nmol/ml和(4.0±0.5)nmol/ml],差异均有统计学意义(P均<0.05).窒息后即刻、高氧1、3和7 d时各组间GSH-Px水平差异均无统计学意义(P>0.05).结论 DHA可能通过提高体内抗氧化酶水平和减少氧化代谢产物,从而对新生鼠窒息后复苏时的高氧性损伤产生一定的保护作用.
목적 탐토모서잉기화포유기보충이십이탄륙희산(DHA)대고양혹공기복소적질식신생대서체내양화화항양화수평적영향.방법 잉서종임신제1천기수궤급여불동음식,종이분위보식조화DHA조(제보통음식외매일급여100 mg DHA).신생서출생24 h내진행결양실험2 h,연후수궤진입고양복소조화공기복소조.실험공분4조:보식+고양복소조、보식+공기복소조、DHA+고양복소조화DHA+공기복소조.용분광광도법분별검측질식후즉각、고양1 d、3 d화7 d시신생서혈장중초양화물기화매(SOD)、병이철(MDA)화곡광감태과양화물매(GSH-Px)수평.결과 DHA+고양복소조신생서재고양1 d、3 d화7 d시적s0D수평분별위(93.7±16.8)U/ml、(128.2±11.5)U/ml화(170.9±9.0)U/ml,현저고우DHA+공기복소조[(73.7±21.5)U/ml、(112.2±19.9)U/ml화(124.2±24.6)U/ml],차이균유통계학의의(P균<0.05).DHA+고양복소조신생서적MDA함량재고양3 d화7 d시분별위(6.0±0.5)nmol/ml화(3.5±0.9)nmol/ml,균현저저우보식+고양복소조[분별위(7.5±1.1)nmol/ml화(4.0±0.5)nmol/ml],차이균유통계학의의(P균<0.05).질식후즉각、고양1、3화7 d시각조간GSH-Px수평차이균무통계학의의(P>0.05).결론 DHA가능통과제고체내항양화매수평화감소양화대사산물,종이대신생서질식후복소시적고양성손상산생일정적보호작용.
Objective To evaluate the impact of docosahexaenoic acid (DHA) on the level of oxidative and ant+oxidative factors in newborn rats with high oxygen resuscitation. Methods Pregnant rats were divided randomly into regular diet group and DHA supplement group (DHA 100 mg/d, Qd, administrated through gavage). Within 24 h after birth, the newborn rats were given low concentration oxygen (8%) for 2 h. Then, the newborn rats were divided into groups exposed to 100% O2 or room air. There were 4 groups: regular diet + hyperoxia-exposed group, regular diet + room air group, DHA + hyperoxia-exposed group and DHA+room air group. The activities of superoxide dismutase (SOD), maleic dialdehyde (MDA) and glutathione peroxidase (GSH-Px) in newborn rats exposed to high oxygen or room air for 1 d, 3 d, 7 d and at the time of immediately after asphyxia were measured by spectrophotometry. Results At day 1,3 and 7, SOD activities in newborn rats of DHA+ hyperoxia-exposed group were (93. 7± 16.8) U/ml, (128.2±11.5) U/ml and (170.9±9.0) U/ml, higher than those in DHA+room air group [(73.7± 21.5) U/mt, (112.2±19. 9) U/ml and (124.2±24. 6) U/ml, respectively] (P<0. 05). At day 3 and 7, the level of MDA in newborn rats of DHA + hyperoxia-exposed group were ( 6.0±0. 5 ) nmol/ml and (3. 5± 0. 9) nmol/ml, lower than those in regular diet + hyperoxia- exposed group [(7. 5±1.1 ) nmol/ml and (4. 0±0. 5) nmol/ml, respectively] (P<0. 05). There were no significant differences of GSH-Px level at any time among the 4 groups (P>0. 05). Conclusions Dietary DHA during pregnancy and breast feeding period might protect newborn rats against hyperoxic damage by increasing antioxidase level and decreasing peroxide level when exposed to high oxygen.