CAJ | 학술논문

目的研究HBV B/C基因型重组方式.方法采用聚合酶链反应扩增4例景洪僾伲族慢性乙型肝炎患者血清中的HBV全基因并与pMD18-T载体相连,转化入大肠杆菌E.coli JM109中,经限制性内切酶酶切鉴定,阳性克隆DNA序列测定后进行HBV基因分型及重组部位的鉴定.结果 4名患者16条HBV全基因均为有不同程度的C基因型毒株重组的B基因型,重组方式有两种:群1与C基因型毒株重组部位只有1个,位于HBV前C/C区nt1825～nt2320,共496 bp;群2与C基因型毒株重组部位有2个,位于P基因区nt822～nt1020和前C/C基因区nt1825～nt2320,共695 bp.结论这种B/C重组方式尚未见报道,Bj亚型与C基因型毒株除了在前C/C基因区重组外,同时还伴有少部分P基因区重组.
목적 연구HBV B/C기인형중조방식.방법 채용취합매련반응확증4례경홍애니족만성을형간염환자혈청중적HBV전기인병여pMD18-T재체상련,전화입대장간균E.coli JM109중,경한제성내절매매절감정,양성극륭DNA서렬측정후진행HBV기인분형급중조부위적감정.결과 4명환자16조HBV전기인균위유불동정도적C기인형독주중조적B기인형,중조방식유량충:군1여C기인형독주중조부위지유1개,위우HBV전C/C구nt1825～nt2320,공496 bp;군2여C기인형독주중조부위유2개,위우P기인구nt822～nt1020화전C/C기인구nt1825～nt2320,공695 bp.결론 저충B/C중조방식상미견보도,Bj아형여C기인형독주제료재전C/C기인구중조외,동시환반유소부분P기인구중조.
Objective To study the recombination type of B/C genotype in hepatitis B virus.Methods The PCR was applied to amplifed the whole genes of HBV through the serums of four chronic HBV carriers who come from Jinghong distict,Yunnan province.The whole HBV genomes were ligated with pMD18-T vector and trasformed to E.coli JM109.After the positive colones were picked up,the HBV genotypes and recombinated sites were discoved through sequenced the acquired positive colones.Results All the acquired sixteen HBV sequences from the four HBV carriers were genotype B which were combinated with genotype C in some region.There are two ways of the combinations.For the first one,a 496 bp fragment from genotype C taked place the genotype B at the place of nt1825 to nt2320 of precore C/C region.For the second way,a 695 bp fragment of genotype B taked place at the both sites of nt822 to nt1020 of P gene region and nt1825 to nt2320 of precore C/C region.Conclusion A new recombination type of B/C genotype in hepatitis B virus was reported for the first time.The new Bj subgenotype was combinated with genotype C not only at the region of precore C/C but also at the place of P gene region.