CAJ | 학술논문

目的观察还原型谷胱甘肽(GSH)对蒿甲醚伍用氯化血红素抗日本血吸虫作用的影响.方法将蒿甲醚、氯化血红素、GSH及谷胱甘肽耗竭剂2,4-二硝基氯苯(CDNB)单一或伍用加入含5周龄血吸虫的培养液内,温育24 h后,测定虫体的丙二醛含量,并观察培养至96 h虫体存活情况.感染小鼠经蒿甲醚300 mg/kg治疗后6、1 2或24h,测定虫体的GSH水平.结果体外,蒿甲醚伍用氯化血红素作用血吸虫24 h后,虫丙二醛含量明显升高.培养时间延长,虫陆续死亡.GSH对蒿甲醚伍用氯化血红素诱导血吸虫脂质过氧化及杀虫作用具有拮抗作用,CDNB则有增强作用.体内,蒿甲醚作用血吸虫6～24 h,虫体内GSH水平先降低再明显升高.结论GSH可能在血吸虫防御蒿甲醚衍生的毒性过氧化物和自由基攻击中起重要作用.
목적관찰환원형곡광감태(GSH)대호갑미오용록화혈홍소항일본혈흡충작용적영향.방법장호갑미、록화혈홍소、GSH급곡광감태모갈제2,4-이초기록분(CDNB)단일혹오용가입함5주령혈흡충적배양액내,온육24 h후,측정충체적병이철함량,병관찰배양지96 h충체존활정황.감염소서경호갑미300 mg/kg치료후6、1 2혹24h,측정충체적GSH수평.결과체외,호갑미오용록화혈홍소작용혈흡충24 h후,충병이철함량명현승고.배양시간연장,충륙속사망.GSH대호갑미오용록화혈홍소유도혈흡충지질과양화급살충작용구유길항작용,CDNB칙유증강작용.체내,호갑미작용혈흡충6～24 h,충체내GSH수평선강저재명현승고.결론GSH가능재혈흡충방어호갑미연생적독성과양화물화자유기공격중기중요작용.
Objective To observe the effect of reduced glutathione (GSH) and glutathione depleting agent 1-chloro2,4-dinitrobenzene (CDNB) on the susceptibility of adult Schistosoma japonicum to artemether (Art) in combination with hemin in vitro. Methods In vitro, malondialdehyde(MDA) levels were determined in five-week-old worms incubated without or with Art, hemin, GSH, and CDNB, either alone or in combination, for 24 h, and the remaining worms were continuously incubated up to 96 h for worm survival assessment. In vivo, GSH levels were determined in worms freshly recovered from mice 6, 12 and 24 h after treatment with Art 300 mg/kg. Results In vitro, GSH decreased the proportion of worms killed by Art plus hemin, but CDNB rendered the worms susceptible to the killing. The above-mentioned distinguishing features of GSH and CDNB were associated with their reverse effect on worm lipid peroxidation induced by Arthemin system. In vivo, Art led to a slight decrease followed by a significant increase in the parasite GSH levels. Conclusion GSH might play an important role in the defense of the worms against Art-generated toxic peroxides and free radicals.