CAJ | 학술논문

利用RAPD分子标记对5个栲树(Castanopsis fargesii Franch.) 天然群体共计188个个体的遗传多样性和群体遗传结构进行了分析.41个随机寡核苷酸引物共检测到385个位点,其中多态位点157个,占40.78%.物种水平的Shannon多样性指数I=0.459 7,Nei基因多样度h=0.296.遗传变异分析表明,栲树群体的遗传变异主要存在于群体内,利用Shannon多样性指数估算的分化(Hsp-Hpop)/Hsp=0.047 6,遗传分化系数Gst =0.042 9,分子方差分析(AMOVA)也证实了这一结论,群体内的变异组分占了94.97%,群体间变异只占5.03%.AMOVA分析结果的显著性检验也表明,群体间及群体内个体间均呈现出显著分化(P<0.001).
이용RAPD분자표기대5개고수(Castanopsis fargesii Franch.) 천연군체공계188개개체적유전다양성화군체유전결구진행료분석.41개수궤과핵감산인물공검측도385개위점,기중다태위점157개,점40.78%.물충수평적Shannon다양성지수I=0.459 7,Nei기인다양도h=0.296.유전변이분석표명,고수군체적유전변이주요존재우군체내,이용Shannon다양성지수고산적분화(Hsp-Hpop)/Hsp=0.047 6,유전분화계수Gst =0.042 9,분자방차분석(AMOVA)야증실료저일결론,군체내적변이조분점료94.97%,군체간변이지점5.03%.AMOVA분석결과적현저성검험야표명,군체간급군체내개체간균정현출현저분화(P<0.001).
Genetic diversity and population genetic structure in 188 individuals from five natural populations of Castanopsis fargesii Franch. were studied by RAPD markers. Three hundred and eighty-five loci were identified with 41 oligonucleotide primers, out of which 157 loci were polymorphic and accounted for 40.78% of total genetic diversity at species level. Shannon's indices of diversity (I) and Nei's gene diversity (h) were 0.459 7 and 0.296 at the species level, respectively. The result showed that genetic variation of C. fargesii populations mainly existed within populations. Genetic differentiation (Hsp-Hpop)/Hsp estimated with Shannon's index of diversity and coefficient of gene differentiation (Gst) were 0.047 6 and 0.042 9 respectively, which were confirmed by the analysis of molecular variance (AMOVA). Therefore, it is apparent that within-population variation accounted for 94.97% and among-populations variation accounted for only 5.03% of the total genetic diversity. AMOVA also indicated that there was significant differentiation among populations as well as among individuals within a population.