CAJ | 학술논문

建立了同时测定中国蜂胶水提物中23种酚类化合物的反相高效液相色谱(RP-HPLC)分析方法.采用ZORBAX Eclipse XDB C_(18)色谱柱(150 mm×4.6 mm×5 μm);流动相:甲醇-0.1%甲酸,梯度洗脱,流速1.0 mL/min,检测波长为256和280 nm,进样量20 μL,柱温35 ℃.各对照品质量浓度与色谱峰面积线性关系良好,具有较好的精确度和重现性,标准加入回收率在93.29%～106.61%之间.采用此法,从河北蜂胶中检测出18种对照品,其中表儿茶素含量最高35.50 mg/g WEP,其次是3,4-二甲氨肉桂酸15.41 mg/g WEP.而云南蜂胶中检测到9种对照品,表儿茶素含量最高11.23 mg/g WEP,其次是白杨素3.61 mg/g WEP,两地蜂胶WEP的HPLC图相似度分别为0.099(256 nm) 和0.194(280 nm),化学成分差异明显.
건립료동시측정중국봉효수제물중23충분류화합물적반상고효액상색보(RP-HPLC)분석방법.채용ZORBAX Eclipse XDB C_(18)색보주(150 mm×4.6 mm×5 μm);류동상:갑순-0.1%갑산,제도세탈,류속1.0 mL/min,검측파장위256화280 nm,진양량20 μL,주온35 ℃.각대조품질량농도여색보봉면적선성관계량호,구유교호적정학도화중현성,표준가입회수솔재93.29%～106.61%지간.채용차법,종하북봉효중검측출18충대조품,기중표인다소함량최고35.50 mg/g WEP,기차시3,4-이갑안육계산15.41 mg/g WEP.이운남봉효중검측도9충대조품,표인다소함량최고11.23 mg/g WEP,기차시백양소3.61 mg/g WEP,량지봉효WEP적HPLC도상사도분별위0.099(256 nm) 화0.194(280 nm),화학성분차이명현.
A reversed phase high-performance liquid chromatography method for the simultaneous analysis of 23 kinds of phenolic components in water extract of porpolis (WEP) was developed. The separation was performed on ZORBAX Eclipse XDB C_(18) column (150 mm×4.6 mm, 5 μm) by gradient elution. The mobile phase consisted of ethanol with 0.1% formic acid at the flow rate of 1.0 mL/min. The detection wavelengths were 256 and 280 nm. The injection volume was 20 μL, and the column temperature was maintained at 35 ℃. The method showed good linear relationship, precision and repeatability. The recoveries were between 93.3% and 106.6%. Eighteen Reference compounds were detected in WEP of Hehei by this method. The content of Catechin was the highest(30.50 mg/g), the next was 3,4-dimethoxycinnamic acid (15.41 mg/g). Nine Reference compounds were detected in WEP of Yunnan. The content of catechin was also the highest (11.23 mg/g), the next was chrysin (15.41 mg/g). Similarity of WEPs between the products of Hebei and Yunnan were 0.099 (256 nm) and 0.194 (280 nm). The chemical compositions of two WEPs were remarkably different.