CAJ | 학술논문

[目的]通过确定12株典型分离株的系统发育地位,初步探讨铁矿尾矿中放线菌生物多样性.[方法]采用酚-氯仿法提取基因组DNA,PCR扩增 16S rDNA并测序后进行系统发育分析.[结果]12株铁矿尾矿放线菌均属于链霉菌属,与进化关系最近的已知菌株同源性为98.7%～100%.[结论]铁矿尾矿中可培养放线菌类群以链霉菌为主,其中存在大量潜在的新种.
[목적]통과학정12주전형분리주적계통발육지위,초보탐토철광미광중방선균생물다양성.[방법]채용분-록방법제취기인조DNA,PCR확증 16S rDNA병측서후진행계통발육분석.[결과]12주철광미광방선균균속우련매균속,여진화관계최근적이지균주동원성위98.7%～100%.[결론]철광미광중가배양방선균류군이련매균위주,기중존재대량잠재적신충.
[Objective] The aim was to discuss the actinobacteria biodiversity of iron-mine tailings by phylogenetic analysis of 12 typical isolates. [Method] The genomic DNAs were extracted by phenol-chloroform method; phylogeny analysis was carried out based on 16S rDNA PCR amplification and sequencing. [Result] The results showed that all the 12 strains belong to the genus Streptomyces sharing 98.7-99.9% similarities with their nearest known neighbors. [Conclusion] Streptomyces is the dominant culturable actinobacteria group of iron mine tailings, in which there are many potential novel species.