CAJ | 학술논문

以分泌抗链霉素(SM)单克隆抗体(mAb)杂交瘤细胞株的建立为基础,应用胶体金免疫层析技术成功研制出SM残留快速检测试纸,并对其敏感性、特异性、重复性、符合性等特性进行了测定.结果表明,检测试纸的检测限为8 μg/L;检测上线为100 μg/L;与双氢链霉素的交叉反应为100%,与其他抗生素类药无交叉反应;其敏感性与ELISA试剂盒相当,与ELISA试剂盒的检测结果符合率为100%;检测试纸在10 min内显示结果.
이분비항련매소(SM)단극륭항체(mAb)잡교류세포주적건립위기출,응용효체금면역층석기술성공연제출SM잔류쾌속검측시지,병대기민감성、특이성、중복성、부합성등특성진행료측정.결과표명,검측시지적검측한위8 μg/L;검측상선위100 μg/L;여쌍경련매소적교차반응위100%,여기타항생소류약무교차반응;기민감성여ELISA시제합상당,여ELISA시제합적검측결과부합솔위100%;검측시지재10 min내현시결과.
Based on the generation of monoclonal antibody(mAb) against Streptomycin(SM),the rapid test strip of SM was developed and its sensitivity,specificity,repeatability and stability were tested respectively. The strip showed that its detection limit was 8 μg/L,it generally had upper 100 μg/L;The strip has 100% cross-reactivity towards dihydrostreptomycin and little or no cross-reactivity towards other antibiotics. It′s sensitivity was as same as that of ELISA-Kit and its coincidence rate was 100% compared with ELISA-Kit. With the strip,the test results were showed in less than 10min,apart from very short time to get test results.