CAJ | 학술논문

[目的]建立一种快速准确鉴定和测定人工抗原偶联比的方法.[方法]通过载体蛋白BSA合成桔青霉素人工抗原,用SDS PAGE电泳法、紫外吸收法、质谱法对其进行鉴定和测定.[结果]经过3种方法的鉴定,桔青霉素人工抗原偶联成功,紫外吸收法测得其偶联比是8.4,质谱法测得其偶联比是6.[结论]对3种供试方法比较发现,质谱法能够快速鉴定人工抗原而且能够准确测定人工抗原的偶联比.
[목적]건립일충쾌속준학감정화측정인공항원우련비적방법.[방법]통과재체단백BSA합성길청매소인공항원,용SDS PAGE전영법、자외흡수법、질보법대기진행감정화측정.[결과]경과3충방법적감정,길청매소인공항원우련성공,자외흡수법측득기우련비시8.4,질보법측득기우련비시6.[결론]대3충공시방법비교발현,질보법능구쾌속감정인공항원이차능구준학측정인공항원적우련비.
[Objective] This study was to establish a method for the rapid and accurate detection of the coupling ratio of artificial antigen. [Method] Artificial synthetic antigen of citrinin (CIT) was first conjugated with vector protein bovine serum albumin (BSA) and then identified by SDS-PAGE electrophoresis, and its coupling ratio was measured by UV absorption and mass spectrometry. [Result] The identification result showed that artificial antigen of CIT was successfully coupled, and the coupling ratio was 8.4 by UV absorption while 6.0 by mass spectrometry. [Conclusion] The comparison experiment showed that mass spectrometry could rapidly identify the artificial antigen and accurately detect its coupling ratio. This study provided basis for the preparation of CIT antigen as well as the establishment of an for enzyme-linked immunosorbent assay (ELISA) for citrinin determination.