生物技术进展
生物技術進展
생물기술진전
2011年
6期
432-435,F0003
,共5页
曾振顺%谭志远%张鲜娇%朱红惠
曾振順%譚誌遠%張鮮嬌%硃紅惠
증진순%담지원%장선교%주홍혜
橙色粘球菌%子实体%生活史
橙色粘毬菌%子實體%生活史
등색점구균%자실체%생활사
Myxococcus fulvus%fruiting body%life history
利用体式显微镜(stereomicroscope,SMC)和扫描电镜(scanning electron microscope,SEM)对橙色粘球菌(Myxococcus fulvus)的子实体形成过程进行为期8d的生长周期观察,揭示橙色粘球菌由营养体逐渐聚集变成子实体的过程;SMC观察得到粘细菌菌落形成聚集以及颜色逐渐变深,并最后形成突起的鲜亮的橙色球状子实体。SEM观察得到明显的群体细胞定向运动以及细胞堆积,粘细菌细胞由杆状营养体向球状粘孢子变化,并最终形成由粘孢子组成的球状子实体。
利用體式顯微鏡(stereomicroscope,SMC)和掃描電鏡(scanning electron microscope,SEM)對橙色粘毬菌(Myxococcus fulvus)的子實體形成過程進行為期8d的生長週期觀察,揭示橙色粘毬菌由營養體逐漸聚集變成子實體的過程;SMC觀察得到粘細菌菌落形成聚集以及顏色逐漸變深,併最後形成突起的鮮亮的橙色毬狀子實體。SEM觀察得到明顯的群體細胞定嚮運動以及細胞堆積,粘細菌細胞由桿狀營養體嚮毬狀粘孢子變化,併最終形成由粘孢子組成的毬狀子實體。
이용체식현미경(stereomicroscope,SMC)화소묘전경(scanning electron microscope,SEM)대등색점구균(Myxococcus fulvus)적자실체형성과정진행위기8d적생장주기관찰,게시등색점구균유영양체축점취집변성자실체적과정;SMC관찰득도점세균균락형성취집이급안색축점변심,병최후형성돌기적선량적등색구상자실체。SEM관찰득도명현적군체세포정향운동이급세포퇴적,점세균세포유간상영양체향구상점포자변화,병최종형성유점포자조성적구상자실체。
Life cycle of Myxococcus fulvus was observed with stereomicroscope (SMC) and scanning electron microscope (SEM) by culture it within eight days. The results revealed the process of vegetative cells moveing thgether to form the fruiting body. SMC observation indicated the swarm of Myxococcus fulvus gathered around to form the fruiting body and the color of them turned into bright orange. SEM observation indicated that a pile of cells moved together toward one diretion and heaped up to build up the huge fruiting body. Also, the vegetation cells turnned into the spherical myxospores.