目的 探讨用高脂高糖高能量饲料喂养加小剂量注射链脲佐菌素(STZ)诱导2型糖尿病肾病大鼠模型的可行性及其肾病特点.方法 选用2个月SD大鼠30只按随机数字表法分成正常组(10只)及造模组(20只).造模组大鼠用高脂高糖饲料喂养10周后,并给予小剂量注射STZ以诱导2型糖尿病模型.大鼠造模成功后继续予高脂高糖饲料喂养2个月.实验结束时,测定24h尿微量白蛋白、血肌酐、尿素氮等指标,并作肾脏组织的PAS染色切片.结果 2型糖尿病模型造模成功组的大鼠体重、胆固醇、甘油三酯、胰岛素水平[(468.7 ±8.8)g,( 1.92±0.27) mmol/L,(1.32±0.34) mmol/L,(38.81 ±5.39) mU/L]均高于正常组[(436.9 ±7.4)g,( 1.16±0.17) mmol/L,(0.8±0.18) mmol/L,(21.43 ±4.19) mU/L,t=9,76,8.08,4.44,8.90,P＜0.01]；注射STZ两周后,造模大鼠的血糖、胰岛素水平、胰岛素抵抗指数[( 19.31±1.55) mmol/L,(31.31±8.60) mU/L,( 26.55±6.33)]均高于正常组[(5.45±0.69) mmol/L,(19.97±3.26) mU/L,(4.82±0.84),t=26.383,3.951,10.719,P＜0.01]；实验结束前糖尿病组大鼠的血糖、胰岛素抵抗指数、尿微量白蛋白、肌酐、尿素氮[( 19.27±1.97) mmol/L,(16.70 ±7.51),(72.49±8.53) mg/24 h,(74.76±8.38)μmol/L,(19.09±4.21) mmol/L]均高于正常组[(5.62±0.65) mmol/L,(5.45±1.33),(15.26±2.20) mg/24h,(40.81±1.97) μmol/L,(9.87±2.13) mmol/L,t=20.96,4.66,20.62,12.50,6.35,P＜0.01],肾组织PAS染色显示糖尿病大鼠肾脏病变严重,出现新月体或肾小球硬化等病灶.结论 长时间的高脂高糖高能量饲料喂养大鼠,并注射小剂量STZ能够诱导出2型糖尿病肾病大鼠模型,其肾脏发生肾小球系膜细胞增生、新月体及肾小球硬化等病理学改变.大鼠伴有多饮、多尿、多食症状,具有大量蛋白尿,高血肌酐及尿素氮的特点.
目的 探討用高脂高糖高能量飼料餵養加小劑量註射鏈脲佐菌素(STZ)誘導2型糖尿病腎病大鼠模型的可行性及其腎病特點.方法 選用2箇月SD大鼠30隻按隨機數字錶法分成正常組(10隻)及造模組(20隻).造模組大鼠用高脂高糖飼料餵養10週後,併給予小劑量註射STZ以誘導2型糖尿病模型.大鼠造模成功後繼續予高脂高糖飼料餵養2箇月.實驗結束時,測定24h尿微量白蛋白、血肌酐、尿素氮等指標,併作腎髒組織的PAS染色切片.結果 2型糖尿病模型造模成功組的大鼠體重、膽固醇、甘油三酯、胰島素水平[(468.7 ±8.8)g,( 1.92±0.27) mmol/L,(1.32±0.34) mmol/L,(38.81 ±5.39) mU/L]均高于正常組[(436.9 ±7.4)g,( 1.16±0.17) mmol/L,(0.8±0.18) mmol/L,(21.43 ±4.19) mU/L,t=9,76,8.08,4.44,8.90,P＜0.01]；註射STZ兩週後,造模大鼠的血糖、胰島素水平、胰島素牴抗指數[( 19.31±1.55) mmol/L,(31.31±8.60) mU/L,( 26.55±6.33)]均高于正常組[(5.45±0.69) mmol/L,(19.97±3.26) mU/L,(4.82±0.84),t=26.383,3.951,10.719,P＜0.01]；實驗結束前糖尿病組大鼠的血糖、胰島素牴抗指數、尿微量白蛋白、肌酐、尿素氮[( 19.27±1.97) mmol/L,(16.70 ±7.51),(72.49±8.53) mg/24 h,(74.76±8.38)μmol/L,(19.09±4.21) mmol/L]均高于正常組[(5.62±0.65) mmol/L,(5.45±1.33),(15.26±2.20) mg/24h,(40.81±1.97) μmol/L,(9.87±2.13) mmol/L,t=20.96,4.66,20.62,12.50,6.35,P＜0.01],腎組織PAS染色顯示糖尿病大鼠腎髒病變嚴重,齣現新月體或腎小毬硬化等病竈.結論 長時間的高脂高糖高能量飼料餵養大鼠,併註射小劑量STZ能夠誘導齣2型糖尿病腎病大鼠模型,其腎髒髮生腎小毬繫膜細胞增生、新月體及腎小毬硬化等病理學改變.大鼠伴有多飲、多尿、多食癥狀,具有大量蛋白尿,高血肌酐及尿素氮的特點.
목적 탐토용고지고당고능량사료위양가소제량주사련뇨좌균소(STZ)유도2형당뇨병신병대서모형적가행성급기신병특점.방법 선용2개월SD대서30지안수궤수자표법분성정상조(10지)급조모조(20지).조모조대서용고지고당사료위양10주후,병급여소제량주사STZ이유도2형당뇨병모형.대서조모성공후계속여고지고당사료위양2개월.실험결속시,측정24h뇨미량백단백、혈기항、뇨소담등지표,병작신장조직적PAS염색절편.결과 2형당뇨병모형조모성공조적대서체중、담고순、감유삼지、이도소수평[(468.7 ±8.8)g,( 1.92±0.27) mmol/L,(1.32±0.34) mmol/L,(38.81 ±5.39) mU/L]균고우정상조[(436.9 ±7.4)g,( 1.16±0.17) mmol/L,(0.8±0.18) mmol/L,(21.43 ±4.19) mU/L,t=9,76,8.08,4.44,8.90,P＜0.01]；주사STZ량주후,조모대서적혈당、이도소수평、이도소저항지수[( 19.31±1.55) mmol/L,(31.31±8.60) mU/L,( 26.55±6.33)]균고우정상조[(5.45±0.69) mmol/L,(19.97±3.26) mU/L,(4.82±0.84),t=26.383,3.951,10.719,P＜0.01]；실험결속전당뇨병조대서적혈당、이도소저항지수、뇨미량백단백、기항、뇨소담[( 19.27±1.97) mmol/L,(16.70 ±7.51),(72.49±8.53) mg/24 h,(74.76±8.38)μmol/L,(19.09±4.21) mmol/L]균고우정상조[(5.62±0.65) mmol/L,(5.45±1.33),(15.26±2.20) mg/24h,(40.81±1.97) μmol/L,(9.87±2.13) mmol/L,t=20.96,4.66,20.62,12.50,6.35,P＜0.01],신조직PAS염색현시당뇨병대서신장병변엄중,출현신월체혹신소구경화등병조.결론 장시간적고지고당고능량사료위양대서,병주사소제량STZ능구유도출2형당뇨병신병대서모형,기신장발생신소구계막세포증생、신월체급신소구경화등병이학개변.대서반유다음、다뇨、다식증상,구유대량단백뇨,고혈기항급뇨소담적특점.
Objective To discuss the feasibility of the method that can be used to induce the model of type 2 diabetic mellitus rat and explore the characteristic of the nephropathy.The rats were fed with the high sucrose,high fat and high energy feed for a long time and then it was injected with the low dose STZ.Methods 30 SD rats were selected and then it was randomly divided them into 2 groups,the control group (10 rats) and the model group (20 rats).The model group was fed with the high calorie feed forl0 weeks to induce insulin resistance and then the rats were induced to type 2 diabetes mellitus by injection of streptozocin (30 mg/kg).The rats of the model group were continually fed with the high calorie feed for 2 months.Before the end of this study,the 24-hours microalbuminuria,serum creatinine and blood urea nitrogen were detected and the periodic acid Schiff staining on the kidney were also measured.Results After the rats of the model group were established,the levels of the bodyweight,the cholesterol,the triglyceride and the insulin were [ (468.7 ± 8.8 ) g,( 1.92 ± 0.27 ) mmol/L,( 1.32 ± 0.34) mmol/L,(38.81 ± 5.39 ) mU/L ] respectively,all of them were higher than the levels in the normal group,which were [ (436.9 ± 7.4) g,(1.16 ±0.17)mmol/L,(0.8 ±0.18)mmo1/L,(21.43 ±4.19)mU/L],respectively( t =9.755,8.077,4.437,8.902,P ＜ 0.01 ).After injection of STZ for 2 weeks,the levels of the blood glucose,the insulin and the insulin resistance of the diabetes mellitus rats were [ ( 19.31 ± 1.55 ) mmol/L,( 31.31 ± 8.60) mU/L,(26.55 ± 6.33) ] respectively,it was higher than levels inf the normal group,which was[ (5.45 ±0.69) mmol/L,( 19.97 ± 3.26) mU/L,(4.82 ± 0.84) ] ( t =26.383,3.951,10.719,P ＜ 0.01 ).Before the end of this study,the levels of the blood glucose,insulin resistance,24-hours microalbuminuria,serum creatinine,blood urea nitrogen of the diabetes mellitus rats were [ ( 19.27 ± 1.97 ) mmol/L,( 16.70 ±7.51 ),(72.49 ± 8.53 ) mg/24 h,( 74.76 ± 8.38 ) μmol/L,( 19.09 ± 4.21 ) mmol/L],it was higher than the levels in the normal group,which were [ (5.62 ±0.65) mmol/L,(5.45 ± 1、33),( 15.26 ±2.20) mg/24 h,(40.81 ± 1.97) μmol/L,(9.87 ±2.13) mmol/L,t =20.961,4.657,20.623,12.495,6.352,P ＜0.01 ].And the pathological changes of the diabetes mellitus rats kidney tissues were the most serious through themethod of periodic acid-Schiff's staining (PAS).Conclusions The model of type 2 diabetic mellitus rat was constructed through the way of feeding the SD rats with high sucrose,high fat and high energy feed for a long time and low dose STZ.The diabetic mellitus rats had the symptom of drinking more,eating more and diuresis,and the character of this model had high levels of albuminuria,serum creatinine,blood urea nitrogen.The incrassated glomerular mesangium,the crescent-shaped focus and the glomerulosclerosis were also observed through the PAS.
