中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2012年
4期
357-359
,共3页
杨莉%侯军良%刘玉珍%高会霞%戴二黑
楊莉%侯軍良%劉玉珍%高會霞%戴二黑
양리%후군량%류옥진%고회하%대이흑
细胞因子诱导的杀伤细胞%树突状细胞%慢性乙型肝炎%白细胞介素12
細胞因子誘導的殺傷細胞%樹突狀細胞%慢性乙型肝炎%白細胞介素12
세포인자유도적살상세포%수돌상세포%만성을형간염%백세포개소12
Cytokine-induced killer cells%Dendritic cell%Chronic hepatitis B%Interleukin-12
目的 研究树突状细胞(DC)疫苗对培养的慢性乙型肝炎(CHB)患者细胞因子诱导的杀伤细胞(CIK)免疫功能的调节作用.方法 采用体外细胞培养的方法培养30例CHB患者CIK细胞,分为DC疫苗组和无DC疫苗组,培养14 d后用流式细胞术检测各组CIK中CD3+ CD4+、CD3+ CD8+及CD3+ CD56+T细胞的所占比例,ELISA方法检测培养上清中白细胞介素-12(IL-12)、γ干扰素(IFN-γ)及白细胞介素-4(IL-4)的浓度.结果 DC疫苗组CD3+ CD4+、CD3+ CD.8+及CD3+ CD56+T细胞所占比例分别为18.27％、64.36％和20.00％,无DC疫苗组则分别为17.79％ (P ＞0.05)、54.69％(t=4.130,P＜0.01)和13.39％(t =5.601,P＜0.01).DC疫苗组的CIK培养上清中IL-12、IL-4及IFN-γ的浓度分别为( 177.82±130.06)、(31.77±9.52)、(86.99±56.30) ng/L,无DC疫苗组分别为(80.83±50.15)ng/L(t =3.811,P＜0.01)、(40.33±19.74) ng/L(t =2.141,P ＜0.05)和(42.07±19.68) ng/L(t =4.125,P＜0.01).结论 CIK细胞培养中加入DC疫苗进行诱导,增强了所培养CIK细胞的杀伤活性.
目的 研究樹突狀細胞(DC)疫苗對培養的慢性乙型肝炎(CHB)患者細胞因子誘導的殺傷細胞(CIK)免疫功能的調節作用.方法 採用體外細胞培養的方法培養30例CHB患者CIK細胞,分為DC疫苗組和無DC疫苗組,培養14 d後用流式細胞術檢測各組CIK中CD3+ CD4+、CD3+ CD8+及CD3+ CD56+T細胞的所佔比例,ELISA方法檢測培養上清中白細胞介素-12(IL-12)、γ榦擾素(IFN-γ)及白細胞介素-4(IL-4)的濃度.結果 DC疫苗組CD3+ CD4+、CD3+ CD.8+及CD3+ CD56+T細胞所佔比例分彆為18.27％、64.36％和20.00％,無DC疫苗組則分彆為17.79％ (P ＞0.05)、54.69％(t=4.130,P＜0.01)和13.39％(t =5.601,P＜0.01).DC疫苗組的CIK培養上清中IL-12、IL-4及IFN-γ的濃度分彆為( 177.82±130.06)、(31.77±9.52)、(86.99±56.30) ng/L,無DC疫苗組分彆為(80.83±50.15)ng/L(t =3.811,P＜0.01)、(40.33±19.74) ng/L(t =2.141,P ＜0.05)和(42.07±19.68) ng/L(t =4.125,P＜0.01).結論 CIK細胞培養中加入DC疫苗進行誘導,增彊瞭所培養CIK細胞的殺傷活性.
목적 연구수돌상세포(DC)역묘대배양적만성을형간염(CHB)환자세포인자유도적살상세포(CIK)면역공능적조절작용.방법 채용체외세포배양적방법배양30례CHB환자CIK세포,분위DC역묘조화무DC역묘조,배양14 d후용류식세포술검측각조CIK중CD3+ CD4+、CD3+ CD8+급CD3+ CD56+T세포적소점비례,ELISA방법검측배양상청중백세포개소-12(IL-12)、γ간우소(IFN-γ)급백세포개소-4(IL-4)적농도.결과 DC역묘조CD3+ CD4+、CD3+ CD.8+급CD3+ CD56+T세포소점비례분별위18.27％、64.36％화20.00％,무DC역묘조칙분별위17.79％ (P ＞0.05)、54.69％(t=4.130,P＜0.01)화13.39％(t =5.601,P＜0.01).DC역묘조적CIK배양상청중IL-12、IL-4급IFN-γ적농도분별위( 177.82±130.06)、(31.77±9.52)、(86.99±56.30) ng/L,무DC역묘조분별위(80.83±50.15)ng/L(t =3.811,P＜0.01)、(40.33±19.74) ng/L(t =2.141,P ＜0.05)화(42.07±19.68) ng/L(t =4.125,P＜0.01).결론 CIK세포배양중가입DC역묘진행유도,증강료소배양CIK세포적살상활성.
Objective To investigate the regulation of the immune function of cytokine-induced killer cells(CIK) by dendritic cell (DC) vaccine in the patients with chronic hepatitis B(CHB) in vitro.Methods CIK cells from 30 patients with CHB were cultured in vitro,and were randomly divided into two groups,DC vaccine-treated group and the control group.After 14 days of culture,the percentages of CD3 + CD4+ T,CD3 +CD8 +T and CD3 +CD56+ T cells among CIKs were analyzed by flow cytometry.The levels of IL-12,IFN-γand IL-6 in cell culture supernatant was detected by ELISA.Results The percentages of CD3 + CD4 + T,CD3 +CD8+T and CD3+ CD56+ T cells were 18.27％,64.36％ and 20.00％ in CIKs in DC vaccine group,and 17.79％ ( P ＞ 0.05 ),54.69％ ( P ＜ 0.01 ) and 13.39％ ( P ＜ 0.01 ) in the control group,respectively.The perentage of CD3 + CD4 + T cells were similar between the two groups ( P ＞ 0.05 ),but for the perentage of CD3 + CD8 +T and C D3 + CD56 + T cells were significantly different between the two groups (t =4.130 and 5.601respectively,Ps ＜ 0.01 ).The concentrations of IL- 12,IL-4 and IFN-γin supernatant were ( 177.82 ± 130.06),(31.77 ± 9.52) and (86.99 ± 56.30) ng/L in DC vaccine-treated group respectively,which were significantly different from those of (80.83 ±50.15) ng/L (t =3.811,P ＜0.01 ),(40.33 ± 19.74) ng/L( t =2.141,P ＜0.05) and (42.07 ± 19.68)ng/L(t =4.125,P ＜0.01) in the control group,respectively.Conclusion DC vaccine could enhance the killing function of CIK cells.
