CAJ | 학술논문

目的初步评价BIOMED-2引物在辅助诊断眼附属器淋巴瘤的应用价值.方法收集63例眼附属器淋巴瘤,均为甲醛固定的石蜡包埋标本,提取基因组DNA并通过扩增管家基因β-actin检测其质量,应用BIOMED-2标准化基因重排检测系统中IgH_B和IgK_B两套多重PCR引物进行Ig基因的PCR扩增,并利用基因扫描技术对扩增产物进行克隆性分析.结果 76.2%(48/63)淋巴瘤石蜡包埋标本的DNA可扩增出300 bp大小的β-actin,适于基因重排检测.IgH_B和IgK_B多重PCR引物的淋巴瘤检出率分别为79.2%(38/48)和68.8%(33/48),二者联合的检出率为91.7%(44/48).结论应用较少的BIOMED-2引物结合基因扫描技术能检测出大多数眼附属器淋巴瘤,对临床病理诊断具有较高的辅助价值.
목적 초보평개BIOMED-2인물재보조진단안부속기림파류적응용개치.방법 수집63례안부속기림파류,균위갑철고정적석사포매표본,제취기인조DNA병통과확증관가기인β-actin검측기질량,응용BIOMED-2표준화기인중배검측계통중IgH_B화IgK_B량투다중PCR인물진행Ig기인적PCR확증,병이용기인소묘기술대확증산물진행극륭성분석.결과 76.2%(48/63)림파류석사포매표본적DNA가확증출300 bp대소적β-actin,괄우기인중배검측.IgH_B화IgK_B다중PCR인물적림파류검출솔분별위79.2%(38/48)화68.8%(33/48),이자연합적검출솔위91.7%(44/48).결론 응용교소적BIOMED-2인물결합기인소묘기술능검측출대다수안부속기림파류,대림상병리진단구유교고적보조개치.
Objective To assess the practical value of BIOMED-2 primers in the diagnosis of ocular adnexal lymphoma by PCR. Methods DNA was extracted from 63 formalin-fixed paraffin-embedded (FFPE) ocular adnexal lymphoma specimens. The DNA quality was evaluated by PCR-based amplification of housekeeping gene β-actin. IgH_B and IgK_B primers of BIOMED-2 standardized clonality analysis system were used to evaluate the immunoglobin gene rearrangements. PCR products were analyzed using capillary electrophoresis and GeneScan software. Results 76.2% (48/63) of FFPE samples produced amplifiable DNA for detection of Ig gene rearrangements. Positive detection rates by BIOMED-2 IgH_B and IgK_B primers were 79. 2% (38/48) and 68. 8% (33/48) , respectively, with a combined positive detection rate of 91. 7% (44/48). Conclusions IgH_B and IgK_R primers of BIOMED-2 are suitable for the detection of clonal rearrangements of Ig gene using FFPE specimens of ocular adnexal lymphomas.