CAJ | 학술논문

目的应用红外光谱三级鉴定技术,分别对小承气汤及其加减配伍规律进行研究.方法采用红外光谱、二阶导数红外光谱和二维相关红外光谱法对小承气汤(Ⅰ方)、小承气合剂(Ⅱ方)和活血承气合剂(Ⅲ方)的提取物进行研究分析.结果一维红外光谱中Ⅲ方提取物与Ⅰ方、Ⅱ方提取物的差别明显,而在一维红外光谱与二阶导数光谱中Ⅰ方与Ⅱ方之间的差异却并不明显,但在二维相关红外光谱中两者差别明显,Ⅲ方中增加了白头翁和败酱草两味药,其提取物的红外光谱与前两种复方的提取物有明显变化,如O-H伸缩振动吸收移至3342 cm -,芳香化合物骨架振动移至1614 cm-1,C-O伸缩振动吸收移至1266 cm-1与1056 cm-1,多出了1199 cm-1,吸收峰指纹区在768 cm-1、603 cm-1出峰.结论一维红外光谱中Ⅲ方提取物与Ⅰ方、Ⅱ方提取物差别明显,而在一维红外光谱与二阶导数光谱中Ⅰ方与Ⅱ方之间的差明并不明显,但可利用二维相关红外光谱最终将二者分开.配伍比例在变化对原方影响明显,会直接影响药效的发挥.
목적 응용홍외광보삼급감정기술,분별대소승기탕급기가감배오규률진행연구.방법 채용홍외광보、이계도수홍외광보화이유상관홍외광보법대소승기탕(Ⅰ방)、소승기합제(Ⅱ방)화활혈승기합제(Ⅲ방)적제취물진행연구분석.결과 일유홍외광보중Ⅲ방제취물여Ⅰ방、Ⅱ방제취물적차별명현,이재일유홍외광보여이계도수광보중Ⅰ방여Ⅱ방지간적차이각병불명현,단재이유상관홍외광보중량자차별명현,Ⅲ방중증가료백두옹화패장초량미약,기제취물적홍외광보여전량충복방적제취물유명현변화,여O-H신축진동흡수이지3342 cm -,방향화합물골가진동이지1614 cm-1,C-O신축진동흡수이지1266 cm-1여1056 cm-1,다출료1199 cm-1,흡수봉지문구재768 cm-1、603 cm-1출봉.결론 일유홍외광보중Ⅲ방제취물여Ⅰ방、Ⅱ방제취물차별명현,이재일유홍외광보여이계도수광보중Ⅰ방여Ⅱ방지간적차명병불명현,단가이용이유상관홍외광보최종장이자분개.배오비례재변화대원방영향명현,회직접영향약효적발휘.
Objective To observe the compatibility of compound mixture of Xiao Chengqi.Methods To study the differences of compatibility of compound mixture of Xiao Chengqi by fourier transform infrared spectroscopy.Results The extracts of decoction Huoxue Chengqi mixture ( Ⅲ ) were significantly different from those of decoction Xiao Chengqi decoction( Ⅰ )and decoction Xiao Chengqi mixture( Ⅱ )in the one dimensional fourier transform infrared (FTIR)spectra,but the difference between decoction Ⅰ and decoction Ⅱ could only be differentiated by the Synchronous two dimensional FTIR spectra.Conclusions Different compatibility proportion in Xiao Chengqi compound mixture provides a new platform for the overall research on compatibility of the traditional Chinese herbs.This new method may prevent the single,unilateral and non-integrated defects,putting emphasis on the holism and interrelationship among the compatible herbs.