中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
12期
1129-1132
,共4页
刘朝军%沈定霞%郭静%王凯飞%李东冬
劉朝軍%瀋定霞%郭靜%王凱飛%李東鼕
류조군%침정하%곽정%왕개비%리동동
凝固酶阴性葡萄球菌%同源分析%16S rRNA%gap基因
凝固酶陰性葡萄毬菌%同源分析%16S rRNA%gap基因
응고매음성포도구균%동원분석%16S rRNA%gap기인
Coagulase negative Staphylococcus%Homology analysis%16S rRNA%gap gene
目的 建立对16种常见凝固酶阴性葡萄球菌(CNS)的快速分子鉴定.方法 用gap基因对16种常见CNS进行扩增和测序,获得序列在GenBank进行比对,构建进化树进行同源分析,同时与16S rRNA基因比较.结果 16种常见CNS中,gap基因相似率介于39% ～ 98%,人葡萄球菌和与人葡萄球菌亚种相似率最高(98%),腐生葡萄球菌与木糖葡萄球菌相似率最低(39％),16SrRNA基因相似率介于96% ～ 99%,至少2种细菌99%以上相似率,最多4种葡萄球菌99%以上相似率；进化树同源分析显示,两种方法在检测缓慢葡萄球菌与松鼠葡萄球菌、产色葡萄球菌与中间葡萄球菌和人葡萄球菌与人葡萄球菌亚种时,都有很高的可信度(99%以上),而对于其他10种细菌,gap基因同源分析有较少的不可靠信度(49%,56%),16S rRNA基因同源分析有较多不可靠信度(43%、43%、50%、56%、63%、65%、76%).结论 gap基因序列分析能够对16种常见CNS进行准确鉴定,同源分析可信度要明显优于16S rRNA基因.
目的 建立對16種常見凝固酶陰性葡萄毬菌(CNS)的快速分子鑒定.方法 用gap基因對16種常見CNS進行擴增和測序,穫得序列在GenBank進行比對,構建進化樹進行同源分析,同時與16S rRNA基因比較.結果 16種常見CNS中,gap基因相似率介于39% ～ 98%,人葡萄毬菌和與人葡萄毬菌亞種相似率最高(98%),腐生葡萄毬菌與木糖葡萄毬菌相似率最低(39％),16SrRNA基因相似率介于96% ～ 99%,至少2種細菌99%以上相似率,最多4種葡萄毬菌99%以上相似率；進化樹同源分析顯示,兩種方法在檢測緩慢葡萄毬菌與鬆鼠葡萄毬菌、產色葡萄毬菌與中間葡萄毬菌和人葡萄毬菌與人葡萄毬菌亞種時,都有很高的可信度(99%以上),而對于其他10種細菌,gap基因同源分析有較少的不可靠信度(49%,56%),16S rRNA基因同源分析有較多不可靠信度(43%、43%、50%、56%、63%、65%、76%).結論 gap基因序列分析能夠對16種常見CNS進行準確鑒定,同源分析可信度要明顯優于16S rRNA基因.
목적 건립대16충상견응고매음성포도구균(CNS)적쾌속분자감정.방법 용gap기인대16충상견CNS진행확증화측서,획득서렬재GenBank진행비대,구건진화수진행동원분석,동시여16S rRNA기인비교.결과 16충상견CNS중,gap기인상사솔개우39% ～ 98%,인포도구균화여인포도구균아충상사솔최고(98%),부생포도구균여목당포도구균상사솔최저(39％),16SrRNA기인상사솔개우96% ～ 99%,지소2충세균99%이상상사솔,최다4충포도구균99%이상상사솔；진화수동원분석현시,량충방법재검측완만포도구균여송서포도구균、산색포도구균여중간포도구균화인포도구균여인포도구균아충시,도유흔고적가신도(99%이상),이대우기타10충세균,gap기인동원분석유교소적불가고신도(49%,56%),16S rRNA기인동원분석유교다불가고신도(43%、43%、50%、56%、63%、65%、76%).결론 gap기인서렬분석능구대16충상견CNS진행준학감정,동원분석가신도요명현우우16S rRNA기인.
Objective To establish the rapid molecular diagnosis of 16 common coagulase negative Staphylococcus(CNS).Methods DNA sequencing of 16 CNS would be obtained with gap gene.After the alignment gap gene sequences which were available in the GenBank,the bacteria were identified with homological alignment and phylogenetic tree,and compared with the 16S rRNA gene.Results The sequence similarity of the gap sequences ranged from 39% to 98% in 16 CNS.There were the highest similarity (98%) between S.hominis and S.hominis subsp,and the lowest(39% ) between S.saprophyticus and S.xylosus.The sequence similarity of the 16S rRNA sequences ranged from 96 to 98%,at least two species of bacteria similar rate of 99% and the most four species similar rate of 99%.Phylogenetic homology analysis showed that it was a high confidence(99% ) in the detection ofS.xylosus and S.lentus,S.chromogenes and S.intermedius,S.hominis and S.hominis subsp,but for 10 other species of bacteria,gap homology analysis has less unreliable confidence(49%,56% ) and 16S rRNA has more unreliable confidence(43%,43%,50%,56%,63%,65%,76% ).Conclusion Analysis of gap sequence could identify 16 CNS timely and accurately,with higher confidence than 16S rRNA.
